解郁1号对抑郁模型大鼠海马区白介素-1β白介素-2白介素-6蛋白表达的影响

Effects of Jieyu 1 Hao Decoction on IL-1β IL-2 IL-6 Protein Expression in c Hippocampus of Depression Rats

目的:观察解郁1号方对抑郁模型大鼠海马区白介素-1β(IL-1β)、白介素-2(IL-2)、白介素-6(IL-6)蛋白表达的影响,探讨该方的免疫调节作用。方法:将SD大鼠60只随机分为正常组、模型组、阿米替林组及解郁1号组,每组15只,应用孤养加慢性轻度不可预见性应激方法制备抑郁大鼠模型,采用免疫组化SABC法检测各组大鼠海马区IL-1β、IL-2、IL-6的蛋白表达。结果:与模型组比较,正常组、中西药组大鼠海马CA3区IL-1β、IL-2、IL-6蛋白阳性表达明显,着色较深,阳性表达计数有显著性差异(P<0.01,或P<0.05);中、西药组间相比,IL-1β、IL-2、IL-6蛋白阳性表达计数无明显差异(P>0.05)。结论:解郁1号可能通过提高海马区IL-1β、IL-2、IL-6蛋白表达起免疫调节功能。

Objective：To investigate the effect of JieYu 1 hao Decoction（JY） ,which was a complex prescription with the function of adjusting spleen and stomach ,on IL- 1β, IL -2, IL -6 protein expression in c hippocampus of depression rats, and explore the immune regulation the JY decoction. Methods ： 60 SD rats were randomly divided into the normal, the model, the Amitriptyline and the JY decoction groups,each with 15. Isolated-living condition and chronic mild unpredictable stressure applied suceessively to set up DEP rat model Immunohistochemical method was used to mensurate IL- 1β ,IL-2 ,IL- 6 protein expression in c hippocampus. Results ： Compared with the model group, the normal group, the JY group and Amitriptyline group in hippocampus CA3 area IL - 1β, IL -2, IL -6 protein expression were obvious, because the color was darker, and the nember was more （P 〈 0. 01 or P 〈 0. 05 ） ; Compared with Amitriptyline group, the nember of IL - 1β, IL - 2, IL - 6 protein expression was no signifi- cant difference （ P 〉 0. 05 ）. Conclusion ： JY decoction increases the IL - 1β, IL - 2, IL - 6 protein expression in hippocampus to regulate immune function probablely.