益气滋肾清利法对CPN大鼠模型肾组织TGF-β_1表达的影响

Effect of the Qi-tonifying Kidney-nourishing and Damp-heat-relieving Method on the Expression of Transforming Growth Factor-β_1 in Nephridial Tissue of CPN Rat Model

目的:探讨益气滋肾清利法对慢性肾盂肾炎(CPN)大鼠模型肾组织转化生长因子β1(TGFβ-1)表达的影响。方法:通过短暂结扎大鼠单侧输尿管和膀胱内接种E.coli O111B4的方法复制CPN模型,分为A组(正常组)、B组(模型组)、C组(中药组)和D组(西药组)。灌胃治疗,其中A、B组生理盐水1mL,C组益气滋肾清利方水煎剂1mL,D组左旋氧氟沙星生理盐水1mL,每日1次,共60天;60天后免疫组化检测肾组织TGFβ-1的表达及RT-PCR检测TGFβ-1mRNA的含量。结果:免疫组化及RT-PCR均显示各组TGFβ-1mRNA表达均较正常组增加,B组明显高于C和D组(P<0.01),但C组与D组无明显差异。结论:益气滋肾清利法可抑制慢性肾盂肾炎(CPN)大鼠模型肾组织TGFβ-1的表达。

Objective：To explore the effect of Qi-tonifylng Kidney-nourishing and Damp-heat-relieving method on the expression of transforming growth factor-β1 （TGF-β1 ） in nephridial tissue of CPN rat model. Methoda： The rats were made to CPN model by the method combining injecting E. coli O111 B4 into urinary bladder with temporary unilateral ureter ligation,and divided into 4 groups randomly：A group（ normal group） ,B group（ model group） ,C group（ Chinese herbal drugs group） ,D group（ antibiotics group）. All groups were given intrngastric administration once a day for 60 days：A and B group were given lmL physiological saline,C group was given lmL Qi-tonifying Kidney-nourishing and Damp-heat-relleving recipes deeoeted with water, D group was given lmL L- Otloxacin fluid. At the end of 60th day,nephridial tissues of each group were collected to detect the expression of TGF-β1 by immunohistochemisty and to examine the quantitation of TGF-β1 mRNA by RT-PCR. Results ： Both immunohistochemisty and RT-PCT resuits display that expressions of TGF-β1mRNA of B,C and D group increased compared with A group;B group was much more than C and D group（P 〈0. 01 ） ,but there was no statistically different between C group and D group. Condusion： Qi-tonifying Kidneynourishing and Damp-heat-relieving method can inhibit the expression of TGF-β1 in nephridial tissue of CPN rat model.