摘要
建立同时测定豆类中4种大豆异黄酮成分含量的高效液相色谱法,探讨了该方法的最佳测定条件,结果显示,70%的乙醇在60℃超声提取40min时,效果最佳。该方法简便、灵敏、准确,适用于豆类中4种大豆异黄酮成分的含量测定和产品质量控制。
The HPLC method for determination of four soybean isoflavones in different beans at the same time is established. The optimal conditions for determination are studied. The results show that the best condition are 70% of the ethanol concentration,60 ℃ of the extraction temperature and extracting for 40 minutes. It is also proved that the HPLC method for determination of four soybean isoflavones in different beans and control of the product quality is simple, sensitive and specific.
出处
《粮食与食品工业》
2009年第3期33-35,39,共4页
Cereal & Food Industry
基金
发光与实时分析重庆市重点实验室(西南大学)(CSTC
2006CA8006)
西南大学校青年基金(SWNUQ
2005008)
重庆市自然科学基金(CSTC
2007BB5370)
