腺梗豨莶化学成分的分离与鉴定

Isolation and identification of the chemical constituents from the whole plant of Siegesbeckia pubescens Makino

目的对中药腺梗豨莶（Siegesbeckia pubescens Makino．）进行化学成分的研究。方法通过硅胶柱色谱、ODS、Sephadex LH-20和制备HPLC等手段分离，利用理化性质和光谱方法鉴定结构。结果分离得到8个化合物，分别鉴定为过氧化麦角固醇（ergosterol peroxide，1）、尿嘧啶（uracil，2）、豆甾-4-烯-3-酮（stigmast-4-en-3-one，3）、豆甾醇（stigmasterol，4）、奇任醇（kirenol，5）、琥珀酸（succinic acid，6）β-谷甾醇（β-sitosterol，7）、胡萝卜苷（daucosterol，8）。结论化合物1—3首次从该属植物中得到。

Objective To isolate and identify the constituents of the whole plant of Siegesbeckia pubescens Makino. Methods The compounds were isolated by chromatography such as silica gel, sephadex LH-20, ODS, HPLC. The structures of these compounds were identified by the physico-chemical analysis and spectroscopic methods. Results Eight compounds were obtained from the petroleum extract, chloroform extract and n-butanol extract of the Siegesbeckia pubescens Makino. The structures were determined as ergosterol peroxide（1）, uracil（2）, stigmast-4-en-one （3）, stigmasterol （4）, kirenol （ 5 ）, succinic acid （6）,β-sitosterol （7）, daucosterol （8）. Conclusions Compounds 1 -3 are isolated from the Siegesbeckia for the first time.