高血糖抑制大鼠载脂蛋白M的表达和分泌

Suppression of Apolipoprotein M Expression and Secretion in Hyperglycemia Rat

目的了解高血糖对SD大鼠载脂蛋白M(apoM)表达和分泌的影响。方法于大鼠尾静脉置管,采用微量注射泵以2.5 ml/h速度连续6 h泵入25%葡萄糖注射液的方法建立生理性高血糖大鼠模型。对照组采用同样方法泵入生理盐水。Dot-blotting法检测血清apoM浓度的变化,Real-Time PCR法检测肝脏apoM mRNA的表达。采用Mann-Whitney检验进行统计分析。结果高糖组大鼠肝脏apoM mRNA的表达较对照组降低54%(P<0.01),血清apoM的浓度较对照下降32.1%,但差异无统计学意义(P>0.05)。结论高血糖可抑制大鼠载脂蛋白M的表达和分泌。

Objective To investigate the effect of glucose on the expression and secretion of apolipoprotein M（apoM） in the rats. Methods Hyperglycemia rat models were induced by 6-hour continuous infusion of 25% glucose solution through tail veins at the speed of 2.5ml per hour by microinfusion pumps. The controls were infused with saline in the same way. The concentration of apoM in the serum was assayed by Dot-blotting and the mRNA levels of apoM in the liver were measured by Real-Time PCR. Comparisons among groups were statistically analyzed by two-tailed Mann-Whitney test. A P value less than 0.05 was considered as statistically significant. Results The blood glucose and insulin levels in hyperglycemia group was 3 times higher than that in the control group. The hepatic apoM mRNA levels were significantly decreased in hyperglycemia rats compared to that in the controls（P〈0.01）. The serum apoM levels of hyperglycemia rats were lower than that in the controls, but without statistic significance （P〉0.05）. Conclusion The expression and secretion of apoM are down-regnlated in hyperglycemia rats.