菊花脑叶片组织培养再生植株的研究

Plant Regeneration in the Tissue Culture of Chrysanthemum nankingense Leaves

[目的]建立菊花脑叶片组织培养再生技术。[方法]以菊花脑叶片作为外植体,以MS为基本培养基,通过添加不同激素设计10种培养基,研究不同种类和浓度的激素组合对菊花脑不定芽和根诱导率的影响,筛选愈伤组织诱导、芽诱导及生根的最佳培养基。[结果]将无菌苗的叶片外植体接种于最佳愈伤组织诱导培养基MS+2.0 mg/L 6-BA+0.2 mg/L NAA,培养15 d可获98.0%的诱导率;之后将带有少量外植体的愈伤组织切下,接种到最佳芽诱导培养基MS+1.0 mg/L 6-BA+0.5 mg/L NAA上培养,可获94.0%的出芽率;然后再将1 cm以上的再生芽转接至最佳生根培养基MS+IBA 0.05 mg/L上生根,生根率达100%;生根25 d以上的幼苗经炼苗和移栽后,成活率在95%以上。[结论]建立了菊花脑叶片组织培养再生植株的技术程序。

[ Objective] The study aimed to establish the high efficient tissue-culture regeneration system for the leaf of Chrysanthemum nankingense. [ Method] With the leaves of C. nankingense as explants and MS medium as basic medium, 10 kinds of media were designed by adding various hormone into MS medium so as to research the effect of various hormone combination and coneentrations on induction rate of adventitious bud and rooting of Chrysantherman nank/ngense leaves, and screen the optimal medium for callus induction, bud induction and rooting. [ Result ] The leaf explants of asepsis seedlings were cultured on the optimum medium of MS＋ 2.0 mg/L 6-BA ＋0.2 mg/L NAA for inducing callus for 15 d, which got the induction of 98.0%, and then the callus with less exp/ant were cadtured on optimal medium of MS ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L NAA for bud induction, which got the budding rate of 94.0%, afterward the regenerated buds with length of over lcm were cultured on optimal rooting medium of MS ＋ IBA 0.05 mg/L, which got rooting rate of 100%, fmally the seedlings that rooted for over 25 d had survival rate of over 95% after hardening and transplanting. [Conclusion] In the experiment, the tissue-culture regeneration technique procedure for C. nankingense leaf was established.