利用微卫星标记对3个绵羊品种肉用性状多态性的研究

Study on Polymorphism of Mutton Performance Traits in 3 Sheep Varieties by Microsatellite Markers

[目的]研究3个绵羊品种肉用性状的多态性。[方法]利用微卫星标记技术研究新疆地区无角陶塞特、萨福克与中国美利奴绵羊群体的遗传多态性，并对所列举微卫星位点的多态性进行统计分析。[结果]所测3个绵羊群体的基因频率分布不均匀，其有效等位基因数在12～15，不同等位基因间片段大小的差异不等。3个品种绵羊群体的变异程度很高，各绵羊品种的杂合度为0．8214-0．9252。所选4个微卫星座位均表现出了高度多态性，多态信息含量（PIC）为0．7923～0．9167，是普遍适合于绵羊肉用生产性状的多态标记。[结论]该研究为进一步寻找与生产性能相关的遗传标记提供了理论依据。

[ Objective ] The aim was to study the polymorphism of mutton perforce traits in 3 sheep varieties. [ Method] The genetic polymorphism of 3 sheep populations of poll dorset sheep and Suffolk sheep from Xinjiang and merino sheep from China were studied by microsatellite marker technology and the polymorphism of the listed microsatellite loci were studied through statistic analysis. [ Result] The gene frequency distribution of the 3 detected sheep populations were not even, the effective number of alleles was between 12 and 15 and the difference on fragment length among different alleles was not same. The variation degree of 3 varieties sheep populations was high and the hetemzygosity of each sheep variety was 0.821 4-0.925 2. Four selected microsatellite loci all showed high polymorphism and the polymorphism information content （PIC） was 0.792 3 -0.916 7, which was the polyrnorphism marker generally suitable for mutton performance traits. [ Conclusion] The research provided the theoretical basis for further searching for genetic marker related with production performance.