摘要
背景:塞来昔布为环氧合酶.2(COX-2)选择性抑制剂,近年研究发现其具有抗肿瘤作用。目的:观察塞来昔布对人结肠癌细胞株HT-29巨噬细胞游走抑制因子(MIF)、抑癌基因蛋白PFEN和凋亡相关蛋白Caspase-3表达的影响,探讨其防治结肠癌可能的分子机制。方法:25、50、100μmol/L塞来昔布分别作用于HT-29细胞24h后,以逆转录聚合酶链反应(RT-PCR)检测HT-29细胞MIFmRNA表达,蛋白质印迹法检测MIF、PTEN、Caspase-3蛋白表达。结果:经25~100μmol/L塞来昔布处理后,HT-29细胞MIFmRNA和蛋白表达随塞来昔布浓度的增加而减低,PTEN和Caspase-3蛋白表达随塞来昔布浓度的增加而增加,与正常对照组相比差异均有统计学意义(P〈0.05)。结论:塞来昔布的抗结肠癌作用可能与下凋MIF表达和上调PTEN、Caspase-3表达有关。
Background: Recent studies demonstrated that celecoxib, a selective inhibitor of cyclooxygenase-2 (COX-2), has anticancer effect. Aims: To investigate the effect of celeeoxib on expressions of macrophage migration-inhibitory factor (MIF), tumor suppressor gene protein PTEN and apoptosis-associated protein Caspase-3 in human colon cancer cell line HT- 29, and its potential molecular mechanism in the prevention and treatment of colon cancer. Methods: Expression of MIF mRNA in HT-29 cells treated witb 25, 50 and 100μmol/L celecoxib, respectively for 24 hours was determined by reverse transcriptase polymerase chain reaction (RT-PCR), and expressions of MIF, PTEN and Caspase-3 proteins were determined by Western blotting. Results: Expressions of MIF mRNA and protein in HT-29 cells treated with 25-100 μmol/L celecoxib were down-regulated in a dose-dependent manner as compared with normal controls (P〈0.05), whereas the expressions of PTEN and Caspase-3 proteins were up-regulated in a dose-dependent manner (P〈0.05). Conclusions: The anti-colon cancer effect of celecoxib might be related to the down-regulation of MIF and up-regulation of PTEN and Caspase-3.
出处
《胃肠病学》
2009年第5期266-269,共4页
Chinese Journal of Gastroenterology
基金
本课题由湖南省科技厅2008年省级科技计划项目资助(2008FJ3088)