摘要
背景：塞来昔布为环氧合酶．2（COX-2）选择性抑制剂，近年研究发现其具有抗肿瘤作用。目的：观察塞来昔布对人结肠癌细胞株HT-29巨噬细胞游走抑制因子（MIF）、抑癌基因蛋白PFEN和凋亡相关蛋白Caspase-3表达的影响，探讨其防治结肠癌可能的分子机制。方法：25、50、100μmol／L塞来昔布分别作用于HT-29细胞24h后，以逆转录聚合酶链反应（RT-PCR）检测HT-29细胞MIFmRNA表达，蛋白质印迹法检测MIF、PTEN、Caspase-3蛋白表达。结果：经25～100μmol/L塞来昔布处理后，HT-29细胞MIFmRNA和蛋白表达随塞来昔布浓度的增加而减低，PTEN和Caspase-3蛋白表达随塞来昔布浓度的增加而增加，与正常对照组相比差异均有统计学意义（P〈0．05）。结论：塞来昔布的抗结肠癌作用可能与下凋MIF表达和上调PTEN、Caspase-3表达有关。
Background： Recent studies demonstrated that celecoxib, a selective inhibitor of cyclooxygenase-2 （COX-2）, has anticancer effect. Aims： To investigate the effect of celeeoxib on expressions of macrophage migration-inhibitory factor （MIF）, tumor suppressor gene protein PTEN and apoptosis-associated protein Caspase-3 in human colon cancer cell line HT- 29, and its potential molecular mechanism in the prevention and treatment of colon cancer. Methods： Expression of MIF mRNA in HT-29 cells treated witb 25, 50 and 100μmol/L celecoxib, respectively for 24 hours was determined by reverse transcriptase polymerase chain reaction （RT-PCR）, and expressions of MIF, PTEN and Caspase-3 proteins were determined by Western blotting. Results： Expressions of MIF mRNA and protein in HT-29 cells treated with 25-100 μmol/L celecoxib were down-regulated in a dose-dependent manner as compared with normal controls （P〈0.05）, whereas the expressions of PTEN and Caspase-3 proteins were up-regulated in a dose-dependent manner （P〈0.05）. Conclusions： The anti-colon cancer effect of celecoxib might be related to the down-regulation of MIF and up-regulation of PTEN and Caspase-3.
出处
《胃肠病学》
2009年第5期266-269,共4页
Chinese Journal of Gastroenterology
基金
本课题由湖南省科技厅2008年省级科技计划项目资助（2008FJ3088）
