大肠癌细胞在硝酸纤维素膜上的培养及染色的观察

Obesrvation of culture and staining of three colon cancer cell lines on nitrocellulose membrane

目的寻找一种既适于细胞培养和光镜下观察,而且具有良好柔韧度及化学稳定性、可替代玻片作为实验室细胞培养载体的支持介质。方法硝酸纤维素膜经前期处理后,用于培养细胞和病理染色并与常规方法培养细胞的比较。结果肉眼观察:染色后的NC膜平展,透明,有浅紫红色的背景。镜下观察:NC膜透明,膜上培养的细胞轮廓清晰,形态规整。细胞形态、数量与对照组玻片培养的细胞无明显差异。HE染色的细胞胞核为蓝色,胞浆红色,膜背景着色较浅。结论经过处理后的NC膜,对细胞无毒副作用,可用于细胞培养。在NC膜上养成的细胞依然保持细胞的正常形态及数量,具有良好的透光性,可满足光镜观察需求。

Objective To establish a new method which use nitrocellulose （NC） membrane as a cell cul- ture carrier to replace coverslips. Methods After simple pretreatment of NC membrane, cell lines were cul- tured on the membrane within culture medium, in parallel with the same cell culture on the coverglass which served as control. Results NC membrane was shown spreading, transparence, edge sharpness companied with purple background with macroscopic observation. Under microscopic observation, there was no significant dif- ference for the cell morphology, cell number between NC membrane culture and coverslip culture. NC mem- branes showed transparence, sharp edge and regular morphology. Fusiform shape of LOVO cells, short rod- shape of SW480 cells and irregular polygon shape of SW1116 cell were found on the NC membrane. In addi- tion, cells were grown in colony pattern and showed blue nucleus and red cytoblasm. Conclusions NC mem- brane via simple pretreatment was no toxicity to the cells, and can be used in cell culture. It not only kept the cell normal morphology and cell number, but also met the transparence for microscope observation.