大鼠脑室系统出血模型的建立

A minimally invasive approach for establishing rat models of intraventricular hemorrhage

目的探讨建立稳定、制作过程简单、创伤小的大鼠脑室系统出血模型的方法。方法取大鼠自体动脉血立体定向下注入右侧侧脑室建立大鼠脑室系统出血模型，对模型组和对照组大鼠在不同时间点进行神经行为学评分，并观察脑室及室周脑组织病理变化。结果本方法模型成功率为88．9％（16／18），注血后6h大鼠出现行为异常，7d后行为异常好转；光镜下观察发现注血后24h模型组大鼠室管膜连续性遭到破坏．细胞间隙增宽，室周脑组织轻度水肿及出现红色坏死神经元。结论本研究采用的造模方法模型稳定．制作创伤小，病理变化接近临床。

Objective To explore a reliable, simple and minimally invasive approach for establishing rat models of intraventricular hemorrhage （IVH）. Methods The rat model of IVH was established by stereotactic injection of autologous arterial blood into the right lateral ventricle. The neurobehavioral scores of the rats were recorded at different time points after the injection, and the pathological changes in the ventricular and periventricular brain tissues were observed. Results WH was successfully induced in 88.9% of the rats, which exhibited significant behavioral changes 6 h after IVH. The behavioral abnormalities were ameliorated 7 days after intraventricular injection of the blood. Optical microscopy showed ruptured continuity of the ependymal lining of the lateral ventricle, enlargement of the intercellular space, periventricular edema and neuronal necrosis in periependymal tissues 24 h after IVH. Conclusion The approach we adopted allows convenient establishment of a stable IVH model in rats with minimal invasiveness and pathological changes closely resembling those in patients with IVH.