摘要
目的了解转染VEGF-ASODN(血管内皮生长因子反义寡核苷酸)对胃癌细胞Survivin(生存素)表达、细胞生长的作用.方法人工合成硫代磷酸化VEGF-ASODN,转染胃癌细胞SGC-7901,用Western-blot法检测细胞Survivin蛋白表达量、用流式细胞仪检测转染后细胞凋亡情况、用MTT检测转染后细胞生存力、细胞生长曲线检测转染后细胞生长情况.结果转染VEGF-ASODN能降低细胞Survivin蛋白,与对照组比较差异有统计学意义(P<0.05),转染后细胞凋亡与对照组比较差异有统计学意义(P<0.05),能降低细胞的生存力及抑制细胞生长.结论VEGF-ASODN能抑制Survivin蛋白表达、抑制胃癌细胞生长、增加胃癌细胞的凋亡.
Objective To observe the effects of VEGF antisense oligonucleotide on expression of survivin and growth in gastric cancer cells. Methods The VEGF-ASODN was synthesised artificially. After transfected with VEGF-ASODN, survivin protein was measured by Western-blot, apoptosis was detected by FCM, survival of cells was detected by MTI＇. Growth of cells was evaluated by growth curve. Results Transfected with VEGF-ASODN, it reduced the expression of survivin remarkably, increased apoptosis and decreased the survival and the growth of the cells. Conclusion VEGF-ASODN can reduce the expression of survivin, suppress the growth and increase the apoptosis of gastric cancer cell.
出处
《昆明医学院学报》
2009年第6期31-34,共4页
Journal of Kunming Medical College
