子痫前期患者腹部皮下脂肪组织和胎盘组织中抵抗素的表达

Expression of resistin in abdominal subcutaneous adipose tissue and placental tissue of patients with pre-eclampsia

目的:探讨抵抗素(resistin)的表达变化在子痫前期发病机制中的作用。方法:收集40例正常妊娠妇女和39例子痫前期患者的腹部皮下脂肪组织和胎盘组织,分别运用RT-PCR技术和Western blot印迹法检测抵抗素mRNA及其蛋白质的表达。结果:与正常妊娠妇女比较,子痫前期患者胎盘组织中抵抗素mRNA及其蛋白质的表达水平均显著降低,差异均有统计学意义(P<0.05)。但是,子痫前期患者腹部皮下脂肪组织中抵抗素mRNA及其蛋白质的表达水平无明显变化,差异均无统计学意义(P>0.05)。此外,两组孕妇胎盘组织中抵抗素mRNA及其蛋白质的表达水平均显著高于腹部皮下脂肪组织,差异均有统计学意义(P<0.05)。结论:胎盘滋养细胞下调抵抗素基因的转录,降低其蛋白质的表达水平,可能在子痫前期的发病机制中发挥重要作用。

Objective To investigate the role of resistin in the pathogenesis of pre-eclampsia. Methods The expressions of resistin mRNA and its protein in abdominal subcutaneous adipose tissue and placental tissue in 40 normal pregnant women （control group） and 39 patients with pre-eclampsia （pre-eclampsia group） were measured by RT-PCR or Western blot. Results As compared with control group, levels of resistin mRNA and its protein in placental tissues were significantly lower in pre-eclampsia group （P 〈 0.05）. But there was no significant difference of that in abdominal subcutaneous adipose tissues between the two groups （P 〉 0.05）. Levels of resistin mRNA and its protein in placental tissues were significantly higher than those in abdominal subcutaneous adipose tissues in both groups （P〈 0.05）. Conclusion Placental trophocytes can reduce the expressions of resistin mRNA and its protein, and may play a significant role in the pathogenesis of pre-eclampsia.