摘要
目的:观察瘦素(leptin)对缺氧复氧人正常肝细胞(L02)凋亡的影响。方法:将L02细胞分别分为正常对照组、单纯缺氧12 h复氧组(IR组)和缺氧12 h复氧加不同浓度的瘦素(分别为100μg/L、200μg/L、400μg/L、800μg/L和1 600μg/L)干预组,以流式细胞仪分析、DNA缺口末端标记(TUNEL)试验、荧光定量PCR等方法观察leptin对L02肝细胞凋亡、Fas/FasLmRNA表达的影响。结果:(1)与正常对照组相比,IR组细胞凋亡率和TUNEL细胞阳性率增加(P<0.01),加用不同浓度瘦素干预组的细胞凋亡率和TUNEL细胞阳性率与IR组相比明显下降(P<0.05);(2)与正常对照组相比,IR组L02细胞中Fas/FasLmRNA表达明显上调(P<0.01);加用不同浓度瘦素干预组与IR组相比,Fas/FasLmRNA表达下降,以400μg/L瘦素作用明显,结果有显著差异(P<0.05)。结论:瘦素能减轻缺氧复氧培养L02肝细胞的凋亡,其机制可能与其下调细胞中Fas/FasLmRNA的表达有关。
AIM: To observe the effect of leptin (LEP) on hypoxia -reoxygenation induced apoptosis in L02 cells. METHODS: In the experiment, L02 cell injury was induced by hypoxic air (95% N2 and 5 % CO2 ). The cultured L02 cells were divided into hypoxic 12 h group (IR group) alone, normal control group and the hypoxic plus leptin (100 μg/L, 200μg/L, 400 μg/L, 800μg/L and 1 600 μg/L) treatment groups in vitro. Flow cytometry, terminal dUTP nick -end labeling (TUNEL) assay and fluorescent quantitative PCR were used to measure the changes of apoptosis in L02 cells and expression of Fas/FasL mRNA. RESULTS : ( 1 ) The percentage of L02 cells apoptosis and positive TUNEL cells significantly increased in IR group compared to control group (P 〈 0.01 ). When L02 cells were treated with LEP, the percentage of cell apoptosis and positive TUNEL cells were decreased compared to IR group. (2) Compared to control group, the Fas/FasL mRNA expression significantly increased in IR group (P 〈0.01 ). When L02 cells were treated with LEP, the Fas/FasL mRNA expression decreased compared to IR group, the effect of LEP at concentration of 400 μg/L was obviously ( P 〈 0.05 ). CONCLUSION : LEP decreases the apoptosis of hypoxic - reoxygenation L02 cells by down - regulation of Fas/FasL mRNA expression in L02 cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第6期1147-1150,共4页
Chinese Journal of Pathophysiology