新疆维、汉族宫颈腺癌与HPV各类型关系的研究

The study of the relationships between HPV subtypes and Xinjiang Uygur and Han with cervical adenocarcinoma

目的:探讨人乳头瘤病毒(Human papilloma virus,HPV)各亚型在新疆维、汉两个民族妇女宫颈腺癌患者中的分布情况及其差异,为新疆宫颈腺癌的防治工作提供理论依据。方法:采用导流杂交基因芯片技术,即凯普导流杂交HPV DNA检测法,对100例新疆宫颈腺癌患者(汉族70例,维族30例)宫颈石蜡包埋组织进行HPV21种亚型的检测。结果:(1)100例宫颈腺癌组织中HPV(包括单一感染及多重感染)总阳性率为61.0%,其中70例汉族患者HPV阳性率为60.0%,30例维族患者HPV阳性率为63.3%,两个民族HPV阳性率差异无统计学意义(P>0.05)。(2)所有标本HPV21种亚型中5种被检测到,16种未检测到,HPV各亚型在两个民族HPV阳性宫颈腺癌中分布分别为:汉族HPV16检出率为90.4%、其次为HPV18(23.8%)、HPV68(4.7%);维族HPV16检出率为89.5%,其次为HPV18(21.0%)、HPV68(10.5%),两个民族HPV16阳性率差异无统计学意义(P>0.05)。(3)HPV阳性率在临床Ⅰ、Ⅱ期和Ⅲ、Ⅳ之间无显著差异(P>0.05)。(4)HPV感染率与肿瘤大小间差异有统计学意义(P<0.01)。结论:宫颈腺癌HPV感染率在维汉两个民族中无差异。维汉两个民族宫颈腺癌均与HPV感染有关,尤其是与HPV16感染有关,除了HPV16和HPV18以外,HPV68是新疆宫颈腺癌中较常见的类型,这与国内外文献报道不同。此外,HPV感染与本组肿瘤大小相关,但与临床分期无关。

Objective： To investigate the distribution of subtypes of human papillomavirus （HPV） between Xinjiang Uygur and Han women with cervical adenocarcinoma, provide basis for the prevention of adenocarcinoma of the cervix in Xinjiang. Methods： To detect the 21 subtypes of HPV from 100 cases with cervi- cal adenocarcinoma （70 cases of Han, 30 cases of Uygur）, by using flow-through hybridization and gene chip technology. Results. （1）The positive rate of HPV in 100 cases of cervical adenocarcinoma （including single and multiple infections） was 61.0% ,the positive rate of HPV in Han was 60.0%, while 63.3% in Uygurs. There has no significant difference between two nations （P〉0.05）. （2）All Specimens of 21 kinds of HPV subtypes were detected in five kinds, 16 species were not detected. The distribution of HPV subtypes in the two nations： HPV16 rate was 90. 4% of Han, followed by HPV18 （23. 8%） and HPV 68（4.7%）; the rate of HPV16 was 89.5% in Uygurs, followed by HPV18 （21%） and HPV68（10.5%）, the positive rate of HPV16 have no significant differences two nations （P〉0.05）. （3）The positive rate of HPV in all patients have no significant difference between clinical stages. （4） There are significant difference between tumor size and The HPV infection rate （P〈0.05）. Conclusions. The cervical adenocarcinomas of both Uygur and Han were closely related to the HPV infection, in particular with HPV16 infection. The next common subtypes of HPV were HPV18 and HPV68. Xinjiang Uygur and Han women with cervical adenocarcinoma had the relatively higher risk of suffering HPV68 infection, this is different from other reports. The HPV infection is related to tumor size, but no related to clinical stages of cervical adenocarcinoma.