新疆哈萨克族食管癌中survivin基因真核表达载体的构建

Construction of survivin in Kazak's esophageal cancer eukaryotic expression vector

目的:探讨survivin基因真核表达载体的构建,研究它在新疆哈萨克族食管癌中的作用。方法:采用PCR方法从新疆哈萨克族食管癌的cDNA表达阳性的标本中扩增出survivin基因,经双酶切后、与真核表达载体pEGFP-N1相连后转入DH5α。结果:DNA测序证明获得了包含ORF全长的survivin基因,并成功克隆入真核表达载体pEGFP-N1中。结论:构建真核表达载体成功,为进一步探讨survivin基因在哈萨克族食管癌中的作用奠定了基础。

Objective： Investigate the construction of survivin in Kazakh＇s esophageal cancer Eukaryotic Expression Vector, for further study on Kazak＇s esophageal cancer. Methods： We cloned survivin gene with PCR from Kazak＇s esophageal cancer in cDNA expressed positiveness, After restrietive endonuelease enzyme EcoR I and BamH I cut, survivin gene link with Eukaryotic Expression Vector pEGFP-N1 and transfer into DH5a. Results： DNA sequence which included ORF was gained, and succusful linked with Eukaryotic Expression Vector pEGFP-N1. Conclusion： We sucussful construct the Eukaryotic Expression Vector, it＇s basic for further studying survivin gene effect on Kazakh＇s esophageal cancer effection.