ZnPcH1-PDT对淋巴瘤细胞杀伤作用的实验研究

Killing Effect of ZnPcH_1-PDT on Lymphoma Cells

本研究探讨新型酞菁类光敏剂ZnPcH1介导的光动力疗法(ZnPcH1-PDT)对淋巴瘤细胞的杀伤作用及其机制。采用人Burkitt淋巴瘤CA46细胞、鼠淋巴瘤P388细胞作为研究对象。应用MTT比色法和细胞集落形成实验检测PDT对细胞的杀伤作用,采用AO/EB荧光染色法、DNA片段化分析、TUNNEL、Annexin-V-FITC/PI双染等方法分析细胞的死亡方式。结果表明:ZnPcH1-PDT对人和鼠淋巴瘤细胞均有杀伤作用,且呈量效关系,但CA46细胞对PDT的敏感程度低于P388细胞(p<0.05)。PDT能够诱导细胞凋亡,且呈时间依赖性。Annexin-V-FITC/PI双染法检测还显示PDT作用后CA46细胞以早期凋亡为主,P388细胞则以早期凋亡和坏死为主。结论:ZnPcH1-PDT能够有效地抑制淋巴瘤细胞增殖,诱导细胞凋亡。

The objective of this study was to investigate the effect of ZnPcH1-PDT on the lymphoma cells and its mechanism. Human Burkittg lymphoma cell line CA46 and mouse lymphoma cell line P388 were selected as objects for study. The killing effect of ZnPcH1 -PDT on cells were assessed by MTT method and colony formation assay; the cell death patterns were analyzed by AO/EB fluorescence stain, TdT-rnediated dUTP nick end labeling （TUNEL）, DNA laddor assay; and the different proportions of each death pattern were determined by Annexin-VFTTC/PI double stains. The results showed that ZnPcH1-PDT displayed anti-proliferation effect on both CA46 cells and P388 cells in dose-dependent manner. CA46 cells were less sensitive to PDT than P388 ceLLs（p 〈 0.05 ）. Furthermore, PDT could induce cell apoptosis in time-dependent manner. The rate of cell apoptosis increased in the PDT-treated cells. The results of Annexin-VFTTC/PI stain indicated that early apoptosis was the main death pattern in the PDT-treated CA46 cells, while early apoptosis and necrosis were the main death model in the PDT-treated P388 cells. It is concluded that ZnPcH1-PDT can effectively inhibit lyrnphorna cell proliferation and induce cell apoptosis.