慢性应激致抑郁模型大鼠红细胞促淋巴细胞增殖变化的研究

The study of red blood cells on lyamphocyte proliferation in CUMS

目的:探讨慢性应激致抑郁模型大鼠红细胞促T、B淋巴细胞增殖变化。方法:将48只大鼠随机分为慢性应激致抑郁模型组(40只)和正常对照组(8只),进行相关行为学指标检测及采用四甲基偶氮唑蓝(MTT)比色法检测两组大鼠红细胞促淋巴细胞增殖反应变化。结果:造模14d后,与对照组相比,模型组大鼠体重增长减慢,大脑系数降低(P<0.01),模型组大鼠水平、垂直运动得分、糖水消耗量、1%蔗糖偏爱百分比均明显降低(P<0.01),而纯水消耗量明显增加(P<0.01)。模型组血浆皮质酮水平在给予慢性应激7d达高峰(P<0.01),应激14d有下降的趋势;模型组大鼠与应激前相比,红细胞促T、B淋巴细胞增殖率降低;且在应激后第7、14、21天后,与对照组相比,差异有统计学意义(P<0.01)。结论:慢性应激可诱发实验大鼠较长时间的行为及活动习性的改变而导致抑郁,此过程中红细胞促淋巴细胞免疫功能受到影响,可为观察应激状况下红细胞免疫功能状况提供新的实验方法。

Objective： Study the changes of rat red blood cells on T,B lyamphocyte proliferation in chronic unpredictable mild stress. Methods： 48 wistar rats were randomly divided into the control group and the depression group. To observe lyamphocyte proliferation changes of the red blood cells by MTT colorimetric and the associated behavioral performance was conducted. Results： Fourteen days after CUMS model, In the depression group, weight and organic coefficient of brain decreased in model group （P 〈0.01）, a signifcantly reduced consumption of sucrose solutions, and increased consumption of pure water as compared with the control group （P〈0.01）. Plasma corticosterone levels peaked at the 7th day but in a declining trend after forteen days. The rate of red blood cells on T, B lymphocyte proliferation in depression group was lower than non-stress at 7, 14, 2t d. Conclusions： The repeated chronic mild stress stimulation can induce long-time changes in acts and activities and decrease the rate of T, B lyamphocyte proliferation in red blood cells, which can provide a new experimental method to observe the red blood cell immune function under the stress.