长期应用葡激酶衍生体对大鼠凝血、纤溶系统影响的研究

Influence of Long-used Staphylokinase Derivative on Hemoagglutinative and Fibrinolytic Systems

本研究探讨葡激酶衍生体(SAKD)对正常和高脂喂养大鼠的凝血及纤溶系统的影响及葡激酶衍生体使用的安全性。将正常和模型大鼠各30只分别分为生理盐水组、SAKD组、重组葡激酶(rSAK)组,按照0.5mg/kg体重给药,3天1次,连续15次,观察出血情况,监测用药前后出血时间(BT)、血小板计数(BPC)、部分活化凝血酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)、纤维蛋白原(Fg)、D-二聚体(D-D)、纤溶酶原活性(PLG)、纤溶酶抑制物活性(PI)。结果发现,正常大鼠中SAKD组有1例轻微出血,rSAK组有2例,但均可自行止血;高脂模型组大鼠中SAKD组有2只轻微出血,rSAK组中有3只轻微出血;各组大鼠的血小板计数、D-D、PLG、PI值无明显改变。SAKD处理的高脂组大鼠的APTT、PT、TT值明显延长,Fg减少(p<0.05),SAKD对正常动物出凝血影响较小,但能够改善高血脂大鼠的高凝血状态。结论:本试验剂量的SAKD具有较好的安全性,长期使用能减轻高脂大鼠血液的高凝症状。

This study was aimed to explore the influence of staphylokinase derivative （SAKD） on the hemoagglutinative and fibrinolytic systems, and to determine the safety of the staphylokinase derivative in application. The normal and model rats each 30 were divided into normal saline, SAKD and rSAK groups. The hemorrhage, blecding time （BT）, blood platelet count （BPC）, activated partial thromboplastin time （APTT）, prothrombin time （ PT）, thrombin time （ TT）, fibronogen （ Fg ）, D-dimer （ D-D ）, plasminogen （PLG） and plasmininhibitor activity （PI） were detected before and after the administration with staphylokinase derivative 0.5 mg/kg body weight, once three days for consecutive 15 days. The results indicated that one case of normal rats with SAKD and two cases of high fat diet model group had mild hemorrhage, all of which showed automatic hemostasis; and 3 cases in rSAK group had mild hemorrhage. And the platelet counting, D-D, PLG and PI in all groups did not significantly change. The rats of high fat diet group treated with SAKD showed the significant extension of APTT, PT and TT times, and the decrease of Fg time （p 〈 0.05）. All the experimental results demonstrated that the influence of SAKD on the hemagglutination of the normal animals was lower, however, which can improve the high-hemagglutination status of the rats with high fat diet. It is concluded that the SAKD at the dosage of this study has the higher safety, which can alleviate the high hemagglutination symptoms of the rats with high fat diet.