不孕症患者的自身免疫抗体分析

Study on the Relationship Between Autoimmune Antibodies and Infertility

目的:探讨不孕症与患者自身免疫抗体的相关性。方法:采用酶联免疫吸附实验(ELISA)检测516例不孕症患者的血清抗精子抗体(ASAb)、抗卵巢抗体(AOAb)、抗子宫内膜抗体(EMAb)、抗绒毛膜促性腺激素抗体(hCGAb)及抗心磷脂抗体(ACAb)。选择125例正常妊娠妇女作为对照组,比较不孕症组与对照组5项抗体的阳性率。结果:不孕症组ASAb,AOAb,EMAb,hCGAb和ACAb的阳性率分别为26.7%,24.8%,25.2%,21.7%和21.3%,明显高于正常对照组4.8%,2.4%,1.6%,3.2%和1.6%(P均﹤0.001);5种抗体联合检测阳性率为38.2%,与分别单独检测这5项抗体的阳性率相比,差异均有统计学意义(P均﹤0.05)。结论:ASAb,AOAb,EMAb,hCGAb和ACAb与不孕症有密切相关性,是引起不孕的重要原因。检测这5项抗体可作为不孕症病因诊断的一项指标。

Objective ：To investigate the relationship between autoimmune antibodies and female infertility. Methods： Serum antisperm antibody （ASAb）,anti-ovarian antibody （AOAb）,anti--endometrial antibody （EMAb）, anti-HCG antibody （hCGAb） and antieardiohpin antibody （ACAb） of 516 female patients with infertility and 125 normal controls （control group） were detected by ELISA methods. Results ：In infertility group,the positive rate of ASAb, AOAb, EMAb, hCGAb and ACAb was 26.7%,24.8%,25.2%,21.7% and 21.3% respectively, which were significantly higher than the rates of control group （4.8%,2.4%, 1.6%,3.2% and 1.6% ）（all P 〈 0.001 ）.The positive rate of combined detection of the five antibodies was 38.2%. There was significant difference between the antibodies combined detection and single antibody detection （all P〈0.05）. Conclusion： There is relationship between ASAb, AOAb, EMAb, hCGAb, ACAb and infertility. Autoimmune antibodies are the main factors affecting infertility.Combined detection of the five antibodies is a valuable method in female infertility.