瀑布兰组织培养快速繁殖技术的研究

Study on tissue culture and micropropagation of Dendrobium pierardii

采用植物组织培养技术,开展瀑布兰的诱导分化﹑芽苗增殖、壮苗培养、生根和移栽等系列研究.结果表明:瀑布兰适宜的诱导分化培养基为:MS+BA2.0 mg/L+NAA0.2 mg/L,其分化率可达69.8%;增殖培养基为:MS+BA3.0 mg/L+NAA0.2 mg/L,培养60 d的增殖系数达到4.9;壮苗培养基为:1/2MS+NAA0.2 mg/L+BA0.5mg/L+香蕉泥10%;生根培养基为:1/2MS+NAA0.5 mg/L;当幼苗长高至3-4 cm,生根5-7条,根长至1-2 cm时,移栽至水草中,成活率达75%以上.

This research adopts the plant tissue culture technology to do a series of study of the Dendrobium pierardii, such as： the induced differentiation, shoots proliferation, seedling cultivation, rooting and transplanting The results showed that： the appropriate medium for the induced differentiation of Dendrobium pierardii is MS ＋ BA 2.0 mg / L ＋ NAA 0.2 mg / L with the differentiation rate of 69.8 percent; the proliferation medium is MS ＋ BA 3.0 mg / L ＋ NAA 0.2 mg/ L with the proliferation of cultured 60d coefficient of 4.9; the seedling medium is 1/2MS ＋ NAA 0.2 mg / L ＋ BA 0.5 mg / L ＋ banana mud 10%; the rooting medium is 1/2MS ＋ NAA 0.5 mg/ L; and when the seedlings grew up to 3- 4cm high with 5-7 footings and the root length grew up tol-2 cm, the Dendrobium pierardii was transplanted to the water, then the survival rate of them reached above 75%.