摘要
利用PCR扩增技术,以金针菇菌丝总DNA为模板,扩增出一条长约750 bp的特异性gpd启动子基因条带(命名为gpd-Fv2),并将其连接到载体上,然后转入大肠杆菌DH5α感受态细胞,筛选出阳性质粒。DNA序列与金针菇gpd启动子的同源性为98%。
First, we amplificated a gene band （named as gpd-Fv2） of 750 bp in length based on the DNA of Flammulina velutipes by using PCR amplification technology. Then we connected the gene band to the carrier and transferred it into the competent cell of DH5α, so positive plasmid was excelled. The homology of DNA sequence and gpd gene promoter of Flammulina velutipes was 98%.
出处
《亚热带农业研究》
2009年第2期137-140,共4页
Subtropical Agriculture Research
关键词
金针菇
gpd启动子
基因克隆
Flammulina velutipes
gpd promoter
gene cloning
