HCV抗原检测与HCV-RNA及HCV抗体检测的比较研究

A comparative investigation on the detection of HCV antigen and the detection of its antibody as well as the HCV-RNA

目的评价HCV抗原检测在临床的应用价值。方法用HCV抗原酶联法检测试剂盒对临床送检HCV-RNA及HCV抗体的血标本进行检测,并分析结果。结果39例HCV-RNA阳性标本中,HCV抗原阳性28例(71.8%);116例HCV-RNA阴性标本中,HCV抗原阳性1例(0.86%);96例HCV抗体阳性标本中HCV抗原阳性47例(48.96%),HCV抗体阴性106例中,HCV抗原均为阴性。结论现有HCV抗原检测试剂盒尚不适合作临床丙型肝炎的筛选,但可作为HCV抗体检测的进一步验证,部分程度地替代HCV-RNA检测。

A comparative study on the detection of HCV antigen and the detection of its antibody as well as the HCV- RNA was made in order to evaluate the value of the HCV antigen detection for the clinical use, in which the results of detecting HCV antigen, HCV antibody and HCV-RNA in blood samples by means of the diagnostic kids were compared. It was found that there were 28 HCV antigen-positive samples in 39 samples of HCV-RNA positive samples （71.8%） and from 116 HCV- RNA negative samples, one HCV antigen positive sample was demonstrated. Meanwhile, in 96 HCV antibody samples, 47 samples were HCV antigen positive （48.96 %）. No HCV antibody negative sample was showed to be HCV antigen positive. It is concluded that HCV antigen detection may not be functionally enough to screen the HCV infection, but it may be used as the eonfirmative test for the detection of HCV antibody and also serve as an alternative method of assay of HCV-RNA detection.