摘要
目的通过观察中药大蒜素(allicin)对人髓系白血病细胞株HL-60增殖、凋亡及bcl-2mRNA表达的影响,探讨大蒜素的作用机制。方法采用MTT法绘制细胞生长曲线;TdT酶介导的原位缺口末端标记法(TUNEL)分析凋亡细胞;RT-PCR法检测大蒜素作用后不同时间段bcl-2mRNA表达水平的变化。结果大蒜素对HL-60细胞增殖具有明显的抑制作用,呈时间和浓度依赖性,并能抑制HL-60细胞集落形成。较低浓度大蒜素即可抑制HL-60细胞增殖。作用24hTUNEL法检测到凋亡细胞;细胞凋亡率呈药物浓度依赖性且使bcl-2/bax下调。大蒜素作用HL-60细胞后,bcl-2mRNA表达水平有不同程度下调,并呈剂量依赖性。结论大蒜素能够有效抑制HL-60细胞增殖,并诱导其凋亡;bcl-2表达水平下调可能参与了该过程。
Aim To investigate the effects of allicin on the proliferation and apoptosis of HL-60 cells, and to explore the underlying mechanism. Methods The cell viability and colony formation were detected by MTT assay. Apoptotie cells were tested by means of TUNEL labeling. RT-PCR was used to analyze the bcl-2 mRNA expressions. Results HL-60 cell growth was significantly inhibited by alliein in dose and time dependent manners. Cell colony formation obviously decreased. The typical hypo-diploid pea J apoptotie peaJ appeared in each dose group. Apoptosis occured in a dose-dependent manner. And its later stages were identified by TUNEL labeling methods respectively, and bcl-2/bax was decreased. The expression of bcl-2 mRNA was down-regulated in a time-dependent manner after treatment with allicin at different time points. Conclusion Allicin effectively inhibits growth and induces apoptosis on HL-60 cells, which may be related with the downregulation of expressions of bcl-2.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第6期790-793,共4页
Chinese Pharmacological Bulletin
