摘要
采用超滤法从癞葡萄水提物中初步分离降血糖活性物质。考察了进料流量、操作压力、操作温度及料液质量浓度等工艺参数对超滤过程中膜通量的影响,并对超滤后两组分的相对分子质量分布及降血糖活性进行了测定。结果表明:用截留相对分子质量为10 000的中空纤维膜对癞葡萄水提物进行超滤分离的最适工艺条件为:进料流量200 L/h、温度25℃、压力0.10 MPa、料液质量浓度20 g/L。超滤有效地将癞葡萄水提物分成两个组分,其中相对质量较小的组分具有显著的降血糖活性,而相对质量较大的组分不具有降血糖活性。
The preliminary separation of aqueous extract of Momordica charantia L. Var. Abbreviata Ser. with hypoglycemic effect was performed by ultrafiltration. The effect flow, operation pressure, operation temperature, concentration of the aqueous extract of input on flux was investigated. The molecular weight distribution and hypoglycemic effect of fractions obtained by ultrafiltrion were compared. The results showed that the optimum conditions of ultrafiltration were concentrations of input flow rate of 200 L/h, temperature of 25 ℃, pressure of 0.10 MPa, concentration of aqueous extract of 20 g/L. The results showed that the fraction with low molecular weight had significant hypoglycemic activity, while the fraction with high molecular weight had no hypoglycemic effect.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第3期342-346,共5页
Journal of Food Science and Biotechnology
基金
无锡市产学研合作项目(DY050012)
关键词
癞葡萄
水提物
超滤
分离
降血糖
Momordica charantia L. Var. Abbreviata Ser.
aqueous extract
ultrafiltration
separation
hypoglycemic effect
