小鼠早期胚胎体外培养技术中几种化学因素的影响

EFFECTS OF SOME FACTORS ON THE DEVELOPMENT OF THE EARLY MOUSE EMBRYOS IN VITRO

与胚胎体外培养有关的一些化学物质，如液体石蜡、ＣＯ２、乙醇和水质等，对小鼠早期胚胎发育有明显的影响．有的液体石蜡对胚胎有毒害作用，这种毒害作用与其比重和所含的硝基萘杂质无关．有的国产液体石蜡适合于做早期胚胎培养之用．培养液中混入乙醇对胚胎发育危害很大，浓度达到０．８％时，胚胎完全不发育，而浓度低于０．０５％时，则无明显影响．酒精厂生产的ＣＯ２粗制品，乙醇含量约为～０．１８％，完全适合于做胚胎培养的ＣＯ２气源．用三蒸水配制的培养液进行胚胎体外培养，可获得良好效果．

he effects of some chemicals involved in cultural procedures of early mouse embryos in vitro,such as liquid paraffin(LP),CO2,alcohol and water were investigated.some of LP are toxic to early mouse embryos cultured in vitro.The harmful toxity of LP on the embryos is not related to its specific gravity and the nitronaphthalene mixed in it.Two products of LP produced by chinese factories were quite fine for the culture of embryos.The cultural medium mixed with alcohol by a higher concentration(≥0.8%)had significantly harmful effects on the development of embryos,all embryos in it would not survive.If the concentration of alcohol in medium decreased to 0.05% or below,it had no visible effects on the development of embryos.It showed that the cultural effects of plastic dishes were rather fine as compared with the siliconized glass dishes.