油菜杂种及亲本指纹图谱构建和杂种纯度鉴定

Fingerprints Construction of Hybrid Parents in Brassica Napus of the Series of Guiza and Its Utilization in Hybrid Purity Test

利用SSR分子标记技术对43份贵杂系列甘蓝型杂交油菜品种(组合)及亲本材料进行研究,筛选出了一批在贵杂系列亲本和杂种上具有较高多态性的SSR引物,构建了贵杂系列油菜亲本及杂交组合的指纹图谱。通过对24份甘蓝型油菜的SSR分析,从100对SSR引物中筛选到21对多态性很强且极易识别的引物,从中筛选出4对核心引物(Na12C06、Na10B04b、0110A05、Na10D03),构建了贵杂系列常用油菜亲本的指纹图谱;对这些亲本组配的19个杂交组合进行SSR分析,从100对引物中筛选到22对适合油菜杂交种的多态性引物,从中选取4对核心引物(Na12C06、Na10D03、Na10E02c、0110A05),构建了贵杂系列常用油菜杂交组合的指纹图谱;利用核心引物组合扩增的方法,可以鉴定油菜杂交组合及其亲本的真实性和纯度。

In the study, morphology method and the SSR marker method were used to analyze 43 brssica napus materials including Guiza series hybrid and their parents etc. Differences between the two methods were discussed. High multi--state property SSR primer were selected used in building DNA fingerprinting database of Guiza series hybrid and their parents. The main results were as follows. 24 brassica napus inbred lines were analyzed by using SSR. Twenty--one pairs out of one hundred pairs of SSR primers were selected to give polymorphic, stable and repeated amplification profiles. Four primers including Na12C06, Na10B04b, 0110A05, Na10D03 were selected to build DNA fingerprinting database of all 24 inbred lines. DNA fingerprint map of 19 brassica napus hybrids were built with 21 pairs SSR primes. These primers also gave polymorphic, stable and repeated amplification profiles. Four primers including Na12C06, Na10D03,Na10E02c,0110A05 were selected to build the DNA fingerprinting database of all 19 brassica napus hybrids. Using primer-in-group method can expand the core primers quickly to identify the purity and reality of brassica hybrids.