人肿瘤坏死因子α衍生物a(hTNFαDa)在溶液中的构象和尿素的变性作用

The Conformation of hTNFαDa in Solution and Urea Denaturation

用二维核磁共振并结合X-光单晶衍射的方法，对hTNFαDa在溶液中的构象和它的稳定性进行了初步的探讨．结果表明，当尿素浓度为5mol·L－1时，hTNFαDa逐步解聚为单体和无规线团；NMR弛豫实验也证实了在尿素作用下，hTNFαDa的构象发生了变化.

Using 2D NMR in combination with X-ray single crystal diffraction method, we have studied preliminary the con formation of the native hTNFαDa and its stability in solu- tion. The results show that hTNFαDa is dissociated into monomer and random coil gradually when the urea concentration is about 5 mol. L-1,and NMR relaxation measurement also confirms this conformation change under the action of urea.