寡核苷酸的卟啉化学修饰及其产物纯化的研究

Chemical Modification of Oligonucleotides by Porphyrins and Their Purification

为探索寡核苷酸的修饰方法,合成了一种卟啉修饰的反义寡核苷酸并对其抑制c-myc靶基因表达的效果进行了初步探索。利用卟啉的1-羟基苯并三氮唑活性酯可将卟啉基团直接修饰在商品化的寡核苷酸片断上。用聚丙烯酰胺凝胶电泳结合红外、紫外测定完成寡核苷酸偶合物的鉴定。与其他类似研究相比,此法简便易行,具有较宽的应用范围。

A kind of porphyrin modified antisense oligodeoxynucleotides was synthesized for the method-ological studies of the modification of oligonucleotides. Its preliminary application in the c-myc gene expres-sion was also presented. The porphyrin moiety was modified on the commercial oligonucleotides through the active 1-hydroxybenzotriazole esters of the porphyrins. The resulting oligonucleotide-porphyrin conjugates were purified and identified by the polyacrylamide gel electrophoresis, the infrared and ultraviolet spectra. Compared with other methods , this strategy is more easily handled and has broad application fields.