摘要
用薄板层析分离GL_7_ACA酰化酶反应的产物和底物,然后用薄层扫描仪进行扫描定量,经过与GL_7_ACA和7_ACA标准品的薄层扫描相比较,得出酶反应液中的7_ACA浓度。
The products and substrates of GL_7_ACA acylase were separated by Thin_Layer chromatography and were quantitativly determinated by Thin_Layer scanning. Compared with the Thin_Layer scanning of the standard 7_ACA and GL_7_ACA,the concentration of 7_ACA in the reaction mixture and the GL_7_ACA acylase's activity were calculated.
出处
《安徽大学学报(自然科学版)》
CAS
1998年第2期108-110,共3页
Journal of Anhui University(Natural Science Edition)
关键词
酰化酶
氨基头孢烷酸
戊二酰基
薄层色谱法
GL_7_ACA acylase, 7_Amidocephalosporanic Acid (7_ACA), Glutaryl_7_Amidocephalosporanic Acid (GL_7_ACA), thin_layer chromatography