薄层色谱法测定戊二酰基-7-氨基头孢烷酸酰化酶的活力

Determination of Activity of Glutaryl_7_Amidocephalosporanic Acid Acylase by Thin_Layer Chromatography

用薄板层析分离ＧＬ＿７＿ＡＣＡ酰化酶反应的产物和底物，然后用薄层扫描仪进行扫描定量，经过与ＧＬ＿７＿ＡＣＡ和７＿ＡＣＡ标准品的薄层扫描相比较，得出酶反应液中的７＿ＡＣＡ浓度。

The products and substrates of GL_7_ACA acylase were separated by Thin_Layer chromatography and were quantitativly determinated by Thin_Layer scanning. Compared with the Thin_Layer scanning of the standard 7_ACA and GL_7_ACA,the concentration of 7_ACA in the reaction mixture and the GL_7_ACA acylase's activity were calculated.