雌二醇对体外培养内皮细胞抗氧化活力的影响

Effects of Estradial on the Anti-oxidation Activity of Cultured Bovine Aortic Endothelial Cells

雌激素对心血管系统的保护作用，是育龄妇女少患此类疾病的重要原因。本文以体外培养的小牛主动脉内皮细胞为实验材料，考察了雌二醇对血管内皮细胞抗氧化系统的影响。结果表明，0．5μg／ml雌二醇作用内皮细胞后，细胞内SOD活性显著增加，与此相对应，细胞内过氧化产物MDA的含量显著降低。提示该浓度的雌二醇能够提高细胞内的抗氧化能力，因而可能防止活性氧对内皮细胞的损伤，而这种损伤往往是动脉粥样硬化的开端。0．05μg／ml雌二醇显示类似的作用趋势，但差异未达到显著水平。5μg／ml雌二醇则对内皮细胞内SOD活性有降低趋势，并显著升高了细胞MDA的含量，表明雌二醇对内皮细胞的作用效果与激素水平有关，同时考察了不同剂量雌激素对内皮细胞老化过程中代谢残留物脂褐素含量的影响，未发现与对照间的显著差异。

To determine the anti - atherosclerosis role of estrogen, we studied the effects of estradial on the anti - oxidation activity of cultured bovine aortic endothelial cells (BAECs) in vitro. SOD activity of BAECs was markedly increased by 0. 5μg/ml of estradial incubation for 24 hours. Intracellular MDA content of the same treated cells was reduced. The changes came to another end when concentration of estradial was increased to 5μg/ml. These results indicated that low dose of estradial may have a protective effect on BAECs and high dose cause to a different direction. We also examined lipofuscin content of differential treated aging BAECs, but no significant changes were found.