用过氧化物酶同工酶对中国葡萄属野生种分类和亲缘关系的研究

A Study on the Taxonomy and Relationship of Wild VitisNative to China with POD Isozyme

用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）对我国葡萄属（Ｖｉｔｉｓ）１２个野生种和变种进行了过氧化物酶（ＰＯＤ）同工酶的研究。结果如下：绘制了葡萄属的基本酶谱，共分离出３５条酶带，用过氧化物酶同工酶酶谱可把供试材料分为三组。综合同工酶谱和聚类分析的结果，认为刺葡萄、秋葡萄、复叶葡萄、瘤枝葡萄和华东葡萄是葡萄属中较原始的类型，山葡萄、燕山葡萄、和麦黄葡萄是较进化的类型，种的分组与进化和地理分布有关。

Peroxidase(POD)was used together with polyacrylamide gel electrophoresis (PAGE) in the tests of 76 clone of 12 wild vitis species and varieties native to China.The results are as follows:①A basic zymogram of POD isozyme was drown based on functional leaves of vitis,and 35 isoPOD bands were found in the zymogram. ②12 species and varieties were divided into 3 groups on the basis of the POD isozyme zymogram.③The analysis of the isozyme zymogram and the cluster analysis suggested that V.davidii,V.romanetii,V.piasezkii and V.psedoreticulata belonged to the primitive category,and V.amurensis,V.yeshanensis and V.adstricta evolutional category.④The grouping and evolution of species was related to their geographical distribution.