摘要
用聚丙烯酰胺凝胶电泳(PAGE)对我国葡萄属(Vitis)12个野生种和变种进行了过氧化物酶(POD)同工酶的研究。结果如下:绘制了葡萄属的基本酶谱,共分离出35条酶带,用过氧化物酶同工酶酶谱可把供试材料分为三组。综合同工酶谱和聚类分析的结果,认为刺葡萄、秋葡萄、复叶葡萄、瘤枝葡萄和华东葡萄是葡萄属中较原始的类型,山葡萄、燕山葡萄、和麦黄葡萄是较进化的类型,种的分组与进化和地理分布有关。
Peroxidase(POD)was used together with polyacrylamide gel electrophoresis (PAGE) in the tests of 76 clone of 12 wild vitis species and varieties native to China.The results are as follows:①A basic zymogram of POD isozyme was drown based on functional leaves of vitis,and 35 isoPOD bands were found in the zymogram. ②12 species and varieties were divided into 3 groups on the basis of the POD isozyme zymogram.③The analysis of the isozyme zymogram and the cluster analysis suggested that V.davidii,V.romanetii,V.piasezkii and V.psedoreticulata belonged to the primitive category,and V.amurensis,V.yeshanensis and V.adstricta evolutional category.④The grouping and evolution of species was related to their geographical distribution.
出处
《华北农学报》
CSCD
北大核心
1998年第2期122-126,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金
关键词
葡萄属
基本酶谱
聚类分析
同工酶
野生种
Vitis
Peroxidase (POD)
Isozyme
Basic zymogram
Cluster analysis