活动性结节病诊断指标的研究

Reseach on Markers in Diagnosis Active Sarcoidosis

为找寻新的结节病诊断及活动性指标，我们用紫外分光法及放射免疫技术分别测定ＳＡＣＥ和ＳＰ的含量，用Ｎｏｒｔｈｅｒｎ杂交测定外周血单个核细胞ＡＣＥｍＲＮＡ的表达。发现，ＳＡＣＥ仍可作为诊断结节病并判断其活动性的指标，并可用于鉴别结核：外周血单个核ＡＣＥｍＲＮＡ及血清ＳＰ含量在结节病中明显降低，ＳＡＣＥ与ＳＰ间无相关性。此二项指标可否成为新的临床指标尚待进一步研究和验证。

To find out new parameters in diagnosing sarcoidosis and its activity, the concentration of SACE, SP and expression of ACE mRNA in peripheral blood mononuclear cells were measured by spectrophotometry,radioimmunoassay, and Northern hybridization,respectively.We found SACE could be used as a marker for diagnosing sarcoidosis and its activity,and for distinguishing it from tuberculosis.The expression of ACE mRNA in mononuclear cells and the concentration of SP were both decreased.There was no relation between SACE and SP.If ACE mRNA and SP could be used as markers in clinical should be researched in future.