牛磺酸对离体大鼠脊髓缺氧/复氧损伤的保护作用

Experimental Study of Taurine Protective Effect on Hypoxia/ Reoxygenation in Isolated Rat Spinal Cord

本文在孵育的离体大鼠脊髓组织切片上，观察牛磺酸对缺氧／复氧损伤的影响。缺氧／复氧损伤引起大鼠脊髓组织ＡＴＰ含量减少和ＬＤＨ漏出增多，ＮＯ生成和ＮＯＳ活性明显增加。Ｌ精氨酸转运Ｖｍａｘ增加９７％（Ｐ＜００１），Ｋｍ值无明显变化。用１０和２０ｍｍｏｌ／Ｌ牛磺酸孵育与单纯缺氧／复氧组比较，显著减轻了缺氧／复氧引起的组织ＡＴＰ减少和ＬＤＨ漏出，ＮＯ生成分别低３４％（Ｐ＜００１）和４０％（Ｐ＜００１），组织Ｌ精氨酸转运Ｖｍａｘ降低３２％（Ｐ＜００１）和３８％（Ｐ＜００１），但仅轻度抑制ＮＯＳ活性增加。提示牛磺酸对Ｌ精氨酸／一氧化氮系统的影响可能是其对缺氧／复氧损伤的神经保护作用的机理之一。

The effect of taurine on hypoxia/reoxygenation(H/R) injury in tissue slices of isolated rat spinal cord was investigated.There are decrease of ATP content,increase of LDH leakage,No production and NO Synthase(NOS)activity were obviously elevated during H/R.Maximum transport velocity(Vmax)of L Arginine(L Arg)is 97% higher(P<0 01),while Michaelis Constant(Km)showed no significant changes.In comparison with H/R group,10 and 20 mmol taurine could significantly alleviate ATP decrease and LDH leakage;NO production reduced 34% (P<0 01) and 40%(P<0 01)respectively.Vmax of L Arg lowered 32%(P<0 01) and 38%(P<0 01).But NOS activity was slightly inhibited.Thus it might be suggested that effect of taurine on L Arg /NO sytem may be one of the nervous protective mechanisms during H/R injury.