三七总皂甙对犬心肌的保护作用

Myocardial Protective Effect of Total Saponins of Panax Notoginsen in Dogs

目的：应用犬低温体外循环灌注模型，评价在心脏停跳液中添加三七总皂甙（ＰＮＳ）的心肌保护作用。方法：实验犬１８只，雌雄不分，随机分为３组（每组６只）。１组为对照组，用冷钾停跳液，２组为ＰＮＳ（５０ｍｇ／Ｌ）小剂量组，３组为ＰＮＳ（１５０ｍｇ／Ｌ）大剂量组。结果：心功能指标恢复百分数，超氧化物歧化酶和脂质过氧化产物丙二醛生成，肌酸激酶及其同工酶活力３组比较：１组缺血再灌注后心功能恢复较差，超氧化物歧化酶明显下降（Ｐ＜０．０１），丙二醛及心肌酶明显升高（Ｐ＜０．０１），２组、３组则变化不明显（Ｐ＞０．０５）。１组与２组、３组比较有显著差异（Ｐ＜０．０１），２、３组间亦有显著差异（Ｐ＜０．０１）。心肌超微结构的保护１组最差，３组最好。结论：在心脏停跳液中加较小剂量的ＰＮＳ具有良好的心肌保护作用，加大剂量效果更佳。

? Objective:To evaluate the myocardial protective effect of total saponins of Panax notoginsen(PNS,a kind of Chinese herb medicine)with cardioplegic solution in dogs. Methods:Eighteen dogs were divided into three groups(n=6 in each group):Group 1,received cold potassium cardioplegic solution(CPCS);group 2,CPCS+PNS(50 mg/L)and group 3,CPCS+PNS(150 mg/L).The dog heart was subjected to 90 minutes of ischemic arrest,and then reperfused for 30 minutes at 28℃.The percentage of the cardiac functional recovery,superoxide dismutase(SOD) activity,malondialdehyde(MDA) production,creatine kinase(CK) and its isoenzyme (CKMB) release were measured before ischemia,90 minutes of ischemia,and 30 minutes of reperfusion.Ultrastructures of the myocardium were examined at the last two time intervals. Results:The results showed that the percentages of the cardiac functional recovery including mean arterial pressure,left ventricular pressure,etc.were less and SOD activity was decreased (p<001),while MDA production and CK,CKMB release were increased (p<001)in group 1 after reperfusion.In contrast,there were no apparent changes in both group 2 and group 3(p>005).The differences were significant between group 1 and other 2 groups (p<001) and also between the latter 2 groups(p<001).Myocardial ultrastructures were well preserved in group 3,but poorly in group 1. Conclusion:This study indicates that small dose of PNS added to the cardioplegic solution has a good myocardial protective effect.Increasing the dosage of PNS shows even better results.