乙型肝炎患者血清前S_1抗原与e抗原及HBV-DNA载量相关性的探讨

Relativity among PreS_1,HBeAg and the level of serum HBV-DNA

目的探讨前S1抗原(PreS1)与e抗原(HBeAg)及HBV-DNA载量相关性,评价PreS1的临床检测意义。方法对160例未经抗病毒治疗的慢性乙型肝炎患者采用实时荧光定量PCR法测定HBV-DNA,ELISA法检测PreS1和HBeAg。结果PreS1在乙型肝炎e抗原(HBeAg)阳性组中检出率为84.8%,高于其在HBeAg阴性组中的检出率35.1%(P<0.01);HBV DNA阳性组中PreS1阳性率为76.6%,显著高于HBV DNA阴性组的53.4%(P<0.05)。随着HBV-DVA载量的升高,PreS1和HBeAg的阳性率也随之升高,在HBV-DNA低载量时,PreS1的阳性率明显高于HBeAg。结论HBV PreS1抗原与具有病毒复制活动性意义的指标(HBeAg和HBV DNA)有良好的相关性,PreS1抗原能够比HBeAg更灵敏地反映HBV复制。

Aim To study the relationship among PreS1 , HBeAg and the level of serum HBV-DNA, and to evaluate the clinical significance of PreS1. Methods 160 patients with chronic hepatitis B were selected. Serum level of HBV-DNA was determined by real-time fluorescence quantity PCR. Serum PreS1 and HBeAg were determined by ELISA. Results The detectable rate of PreSl antigen in positive HBeAg group （ 84.8% ） was higher than that in negative HBeAg group （35.1% ） （ P 〈 0.01 ）. The detectable rate of PreS1 antigen in positive HBV DNA group （76.6%） was higher than that in negative HBV DNA group （53.4%） （P 〈 0.01 ）. With the increase in the level of HBV-DNA, the positive rates of PreS1 and HBeAg increased. When HBV-DNA had a low load capacity,the PreS1 masculine gender rate obviously was higher than HBeAg. Conclusion There is a significant correlation between PreSl , HBV DNA load and HBeAg. PreS1 is more sensitive than HBeAg to reflect the HBV replication in chronic hepatitis B patients.