摘要
目的研究利多卡因复合氯胺酮对全脑缺血，再灌注大鼠海马CA1区细胞坏死和凋亡的影响。方法Wistar大鼠60只，随机分为6组：对照组（Ⅰ组，n=4）、假手术组（Ⅱ组，n=4）、模型组（Ⅲ组，n=4）、利多卡因组（Ⅳ组，n=16）、氯胺酮组（Ⅴ组，n=16）、利多卡因复合氯胺酮组（Ⅵ组，n=16），采用四血管阻断法制备全脑缺血，再灌注模型。Ⅳ、Ⅴ、Ⅵ组在夹闭血管前15min分别腹腔注射利多卡因10mg／kg、氯胺酮10mg／kg或利多卡因复合氯胺酮混合液10mg／kg。再灌注12、24、48、72h后行HE染色和细胞凋亡（TUNEL法）检测，观察大鼠海马CA1区细胞坏死和凋亡。结果与Ⅱ组比较，Ⅳ、Ⅴ、Ⅵ组24h缺血神经元显著增多，差异有统计学意义（P〈0．05）；Ⅵ组缺血神经元较Ⅳ、Ⅴ组显著减少，差异有统计学意义（P〈0．05），Ⅳ、Ⅴ组间无统计学差异，缺血神经元高峰在24h出现。与Ⅱ组相比，Ⅳ、Ⅴ、Ⅵ组24h凋亡细胞显著增多，差异有统计学意义（P〈0．05），Ⅵ组细胞凋亡数较Ⅳ、Ⅴ组减少，差异有统计学意义（P〈0．05）；Ⅳ、Ⅴ组间无统计学差异，细胞凋亡高峰在24h到48h间，此后，随再灌注时间的延长而减少。结论利多卡因复合氯胺酮可减少和降低脑缺血／再灌注后大鼠神经细胞坏死和凋亡的发生。
Objective To investigate the effect of lidocaine combined ketamine to the cells necrosis and apoptosis in the CA1 region of rat hippocampus following global cerebral ischemia-reperfusion. Methods Sixty adult Wistar rats were randomly divided into 6 groups：control group（Ⅰ, n=4）,sham operation group （Ⅱ, n=4）, model group （Ⅲ, n =4）, lidocaine group （Ⅳ, n =16）, ketamine group（Ⅴ, n=16）, lidocaine and ketamine group （Ⅵ, n=16）. The global cerebral ischemia （ 10 min） was induced by the use of the four-vessel occlusion method. Group Ⅳ,Ⅴ,Ⅵ intraperitoneally injected the lidocaine 10mg/kg, ketamine 10 mg/kg or lidocaine combined ketamine 10 mg/kg. The effect of cells necrosis and apoptosis was detected by using HE staining and TUNEL methods. Results Compared with group Ⅱ the numbers of ischemia neuron of group Ⅳ, Ⅴ, Ⅵ had significant deviation （P〈0.05） in 24 h, and group Ⅵ had significant decreased than group Ⅳ,Ⅴ（P〈0.05）. The ischemia neurons peak presented in 24 h. Compared with group Ⅱ the numbers of apoptosis of group Ⅳ, Ⅴ, Ⅵ had significantly deviation （P〈0.05）in 24 h, and group Ⅶ had significantly decreased than group Ⅳ,Ⅴ （P〈0.05）. The apoptosis peak presented in 24 h and 48 h, and decreased during reperfusion time. Conclusion Lidocaine combined ketamine can reduce the cell necrosis and apoptosis after global crebral ischemia-reperfusion in rats hippocampus.
出处
《国际麻醉学与复苏杂志》
CAS
2009年第3期206-209,共4页
International Journal of Anesthesiology and Resuscitation
