同种异体移植肾组织中B7蛋白的表达及其意义

The characteristics of B7 expression in allograft biopsies of acute cellular rejection in renal transplantation

目的观察同种异体移植肾组织中Ｂ７蛋白表达的特点，以期阐明其在急性细胞性排异中可能的致病作用。方法用ＰＡＰ免疫组织化学方法对急性细胞性排异（ＡＣＲ），无急性细胞性排异（Ｎ－ＡＣＲ）和正常对照肾组织中Ｂ７蛋白的表达进行观察，并结合肾间质中浸润淋巴细胞数和肾小管上ＨＬＡ－ＤＲ抗原的表达进行分析。结果ＡＣＲ组肾间质ＣＤ４＋，ＣＤ８＋和Ｂ细胞数较Ｎ－ＡＣＲ组和正常对照明显增高，与此相一致的是肾间质表达Ｂ７－１和Ｂ７－２的细胞也较Ｎ－ＡＣＲ组增高，但Ｂ７－２增加更明显。ＡＣＲ组肾小管上皮细胞ＨＬＡ－ＤＲ及Ｂ７－１的表达较Ｎ－ＡＣＲ组明显增高。结论肾小管上皮细胞有可能通过ＨＬＡ－ＤＲ和Ｂ７－１表达的上调作为抗原提呈细胞主动参与上述免疫反应的发生。

Objective To study the characteristic of B7 expression in allograft biopsies of acute cellular rejection in renal transplantation and to investigate the possible pathogenic role of B7 in acute cellular rejection. Methods Patients with acute cellular rejection(ACR,n=12) and those without acute rejection(NACR,n=12)were used in this study.Cadaveric donors of normal kidney(n=10)served as controls.The expression of B71 and B72 in renaltissue was analyzed by immunohistochemistry of PAP method.The data was interpreted in the light of infiltrating lymphocytes in the interstitium and the expression of HLADR on tubular cells. Results[WT5”BZ] A dramatic increment of B72 and a mild augmentation of B71 expression on interstitial cells were found in ACR as compared to NACR,which were in accord with the increment of lymphocytes infiltration in the interstitium.The expression of B71 and HLADR on tubular cell was significantly higher in ACR than NACR,although upregulation of B71 and B72 expression on tubular cells were also seen in NACR cases. Conclusion The costimulatory molecules mediated T cell activation may play an important role in the pathogenesis of ACR.The interstitial infiltration and tubular cells may both act as an antigen presenting cells in the initiation of immune injury in ACR.