四川不同地区硬头黄竹RAPD和ISSR分析

RAPD and ISSR Analysis of Bambusa rigida from Seven Different Regions in Sichuan Province

以四川不同地区硬头黄为研究对象，通过随机扩增多态性DNA标记（RAPD）和简单序列重复区间（ISSR）标记进行扩增，探讨硬头黄竹的遗传多样性。研究表明，6个RAPD引物扩增出42条条带，多态性高达69．05％；5个ISSR引物扩增出47条条带，多态性为61．70％。利用Popgen32和NTYS软件进行聚类分析，可将不同地区硬头黄竹类型区分开来，其遗传距离分别为0．1823～0．6022和0．0435～0．6721，RAPD和ISSR分子标记为硬头黄竹的遗传改良提供理论依据。

The genetic diversity of Barnbusa rigida from seven different regions in Sichuan province was studied using random amplified polymorphic DNA（RAPD） and inter-simple sequence repeat （ISSR） amplification markers. The results showed that 6 RAPD and 5 ISSR primers generated 42 and 47 bands, of which the percentage of polymorphic bands （PPB） was 69. 05% for RAPDs and 61. 70% for ISSRs. The UPGMA analysis of Popgen32 and NTYS program showed the following results： the genetic distance ranged from 0. 1823 to 0. 6022 for RAPDs and 0. 0435 to 0. 6721 for ISSRs. The techniques of RAPD and ISSR molecular markers provided a theoretical basis for the genetic improvement of Bambusa rigida.