经皮肝内盐酸注射消融的有效性及安全性的实验研究

Experimental study of the efficacy and safety of percutaneous hydrochloric acid injection in new zealand rabbits liver

目的研究观察不同浓度,不同剂量盐酸肝内注射后产生的消融范围及其对血生化的影响,并与无水乙醇对比,评价其替代无水乙醇作为肝癌瘤内注射制剂的应用前景。方法新西兰白兔36只。随机分为A、B、C、D4个组。每组9只,A组为18%盐酸组,B组为9%盐酸组,C组为4.5%盐酸组,D组无水乙醇组。再根据注射剂量将每组9只随机分配到a、b、c3个亚组,每亚组3只,a亚组0.5ml,b亚组为1.0ml,c亚组为1.5ml,每只动物肝脏内只进行一次药液注射。结果盐酸注射后造成的坏死区域呈类圆形,边界清晰。随着浓度的增加,剂量的增大,坏死区域也随着增大。注射前后血生化无明显变化。结论盐酸溶液肝内注射所产生的肝组织坏死比无水乙醇更为彻底,且更安全,可以成为理想瘤内注射制剂的一种选择。

Objective To observe the effect of different concentration and doses of hydrechlorie acid injection in rabbit liver on blood bio- chemical parameters, compare hydrochloric acid injection with percutaneous ethanol injection and evaluate the application of intratumoral hydro- ehlorie acid injectionfor for the treatment of hepatoma. Methods Thirty - six New Zealand rabbits were divided into 4 groups at random with 9 rabbits in each group. Group A received hydrochloric acid injection at concentration of 18%, while group B at 9% and group C at 4.5%. Group D received anhydrous alcohol. Nine rabbits of each group were further divided into subgroup a, b and c at random with 3 rabbits in each subgroup. Subgroup a, b and C received injection doses at 0.5 ml, 1.0 ml and 1.5 ml respectively. Each rabbit received injection just one time. Results The necrosis region caused by the injection of hydrochloric acid were round with clear border. The zone of necrosis expanded with the increase of the eoneentratian and the doses injected. No significant difference was observed in blood biochemical parameters before and after injection. Conclusion The necrosis caused by the intrabepatie hydrochloric injection was thorough. The injection is safer than ethanol injection and may become an ideal method for intratumoral injection.