摘要
目的研究HBV感染在小儿膜性肾炎的致病作用。方法32例肾穿刺活组织检查诊为膜性肾炎的小儿作为研究对象。所有病例检测血清HBV感染标志物,29例应用免疫组化技术检测肾组织HBV抗原,12例应用Southern印迹杂交检测肾组织HBVDNA,采用32Pα-dCTP标记的探针;28例应用原位杂交检测肾组织HBVDNA,采用地高辛标记的探针。结果三种方法综合分析,32例膜性肾炎中,肾组织HBV抗原和(或)HBVDNA阳性者共30例,达93.74%。
Objective To investigate the role of HBV in the pathogensis of childhood membranous glomerulonephritis (MGN).Methods 32 cases of MGN were studiedSerum markers of HBV infection were determined in all cases,29 of them with HBV antigenemia(9062%)HBV antigens were detected by immunohistochemical techniques in renal tissue in 24 out of 29 casesHBV DNA were examined in 12 cases by Southern blot hybridization with32 PαdCTP labelled HBV DNA probe,HBV DNA was present in 11 cases (9166%), 9 of them were identified as integrated form while 2 cases were of nonreplicating free formIn addition,HBV DNA were observed in 28 cases by in situ hybridization using digoxigenin labelled HBV DNA prode,in 26 of them HBV DNA was detectable (9285%)in the nucleus of glomerular epithelial cells,mesangial cells and matrix,in 13 cases it was simultaneously found in the nucleus of tubular epithelial cellsResults There were 30 cases of MGN showing positive results for HBV antigen and/or HBV DNA in renal tissue(9374%).Conclusion MGN in children is closely related to HBV infection in China
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1998年第3期159-162,共4页
Chinese Journal of Nephrology