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乙型肝炎病毒感染与小儿膜性肾炎 被引量:11

HBV infection and childhood membranous glomerulonephritis
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摘要 目的研究HBV感染在小儿膜性肾炎的致病作用。方法32例肾穿刺活组织检查诊为膜性肾炎的小儿作为研究对象。所有病例检测血清HBV感染标志物,29例应用免疫组化技术检测肾组织HBV抗原,12例应用Southern印迹杂交检测肾组织HBVDNA,采用32Pα-dCTP标记的探针;28例应用原位杂交检测肾组织HBVDNA,采用地高辛标记的探针。结果三种方法综合分析,32例膜性肾炎中,肾组织HBV抗原和(或)HBVDNA阳性者共30例,达93.74%。 Objective To investigate the role of HBV in the pathogensis of childhood membranous glomerulonephritis (MGN).Methods 32 cases of MGN were studiedSerum markers of HBV infection were determined in all cases,29 of them with HBV antigenemia(9062%)HBV antigens were detected by immunohistochemical techniques in renal tissue in 24 out of 29 casesHBV DNA were examined in 12 cases by Southern blot hybridization with32 PαdCTP labelled HBV DNA probe,HBV DNA was present in 11 cases (9166%), 9 of them were identified as integrated form while 2 cases were of nonreplicating free formIn addition,HBV DNA were observed in 28 cases by in situ hybridization using digoxigenin labelled HBV DNA prode,in 26 of them HBV DNA was detectable (9285%)in the nucleus of glomerular epithelial cells,mesangial cells and matrix,in 13 cases it was simultaneously found in the nucleus of tubular epithelial cellsResults There were 30 cases of MGN showing positive results for HBV antigen and/or HBV DNA in renal tissue(9374%).Conclusion MGN in children is closely related to HBV infection in China
出处 《中华肾脏病杂志》 CAS CSCD 北大核心 1998年第3期159-162,共4页 Chinese Journal of Nephrology
关键词 肾炎 膜性肾炎 乙型肝炎病毒 感染 儿童 Membranous glomerulonephritis HBV antigen HBV DNA
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参考文献6

  • 1马学玲,临床与实验病理学杂志,1996年,12卷,84页
  • 2周士东,中华病理学杂志,1995年,24卷,296页
  • 3陈国柱,中华医学杂志,1995年,75卷,540页
  • 4方利君,中华肾脏病杂志,1992年,8卷,65页
  • 5郭怡清,中华肾脏病杂志,1990年,6卷,82页
  • 6应伟中,中华内科杂志,1984年,23卷,145页

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