肿瘤坏死因子促α粒子致细胞恶性转化的研究

RESEARCH ON THE PROMOTING EFFECT OF TUMOR NECROSIS FXCTOR α IN CELL TRANSFORMATION INDUCED BY α-PARTICLES

实验以0．5Gy^(238)Pu源α粒子照射后的叙利亚地鼠胚胎细胞为靶细胞，10d克隆形成实验评价人重组肿瘤坏死因子α促癌作用。通过传代的方法获得不同代龄的细胞，测定它们的生长速率、锚着独立性生长、裸鼠成瘤性等多项生物学指标。结果表明，人重组肿瘤坏死因子α使0．5Gyα粒子致叙利亚地鼠胚胎细胞转化频率增高2倍；经人重组肿瘤坏死因子α处理的0．5Gyα粒子照射后的叙利亚地鼠胚胎细胞寿命明显延长，第40代细胞对血清的依赖性显著降低，增殖能力增强，并能在半固体琼脂培养基上形成克隆，第40代细胞及分离出的两株单克隆细胞均能在裸鼠体内形成低分化的纤维肉瘤。

In our experiments, syrian hamaster embryo cells (SHE) exposed to ^(238)Pu α particles acted as the target cells, the transformed frequency (TF) was determined by means of 10 days colony formation assay for appraising the promoting effect of recombinant human tumor necrosis factor α (rhTNF-α). Different passage cells were achived by a series of passaging experiments, and their growth kinetics, anchorage independence growth nature and tumorigeniciey in nude mice were analysed. The results showed that (1) rhTNF-α could increzised the TF of primary SHE cells previously irradiated by α 0.5Gy dose of α particles by a factor of two; (2) addition of rhTNF-α to subcultures of irradeated SHE cells permitted the continuous propagation of primary cells up to the both passages;(3) cells from 40th passage sustained growth under low serum condition; and (4) the monoclonal cells Tc, Td and cells of 40th passage were tested for their tumorigenicity in nude mice, and poorly differentiated fibrosarcoma were founed in two out of three nude mice in each group.