维拉帕米逆转KB_(V200)的实验研究

Study of Reversal of KB v200 by Verapamine

目的：研究维拉帕米逆转肿瘤多药抗药性的作用及机理。方法：ＭＴＴ法测药敏；用流式细胞仪测细胞对罗丹明１２３的蓄积、外排及耐药蛋白的表达。结果：耐药株的耐药性是亲本株的１７２倍；维拉帕米使耐药株的ＩＣ５０逆转了２～７９倍。耐药株表达耐药蛋白，亲本株为阴性；维拉帕米不影响耐药蛋白的表达。耐药株与亲本株相比，罗丹明的蓄积减少、外排加速；维拉帕米使耐药株的外排显著延缓。结论：维拉帕米能通过调节耐药蛋白的功能显著地逆转ＫＢｖ２００的多药抗药性。

Objective:This paper was to study the role and mechanism in the reversal of multidrug resistance to cancer cell of Verapamine. Methods:MTT method was used to assay drug sensitivity. We used flow cytometry to measure intracellular accumulation and efflux of Rhodamine 123, and the expression of P glycoprotein. Results: The resistance to Vinblastine was 172 fold higher in KB v200 as compared with that to parent cell. Verapamine and Vinblastine decreased the IC 50 of KB v200 by 2 to 79 fold as did Vinblanstine solely. The expression of P glycoprotein was positive in KB v200 , but negative in parent cell. Verapamine exerted no effect on these process. The accumulation and efflux of Rhodamine 123 was decreased and accelerated respectively in KB v200 as compared with parent cell Verapamine obviously slow the efflux of Rhodamine123 in KB v200 . Conclusions: Verapamine had apparent effect on the reversal of multidrug resistance by modulating the function of P glycoprotein but did not change the expression of this protein.