重组人造血生长因子对抗白血病药物作用的影响

Effects of hematopoietic growth factor on the anti leukemia drugs to acute myeloid leukemia cells in vitro

采用流式细胞仪及ＩＮＴ细胞毒检测，研究造血生长因子（ｒｈＨＧＦ）对抗白血病药物细胞毒性的影响，并探讨ＨＧＦ与化疗药物联合应用的作用机理。研究结果表明：３种抗白血病药物柔红霉素（ＤＮＲ）、三尖杉酯碱（Ｈ）、足叶乙甙（ＶＰ１６）对白血病细胞敏感性存在较大差异；ＧＭＣＳＦ、ＩＬ３均能增加ＶＰ１６、ＤＮＲ对白血病细胞的杀伤作用；ＧＭＣＳＦ能提高Ｈ的细胞毒性作用，而对正常骨髓细胞。

To explore whether rhHGF and chemotherpeutic drugs combined treatment is helpful to killing more AML cells,an in vitro study on the combined effects of G CSF, GM CSF or IL 3 plus the chemotherapeutic drugs(DNR VP 16 or H)on the proliferation and cytotoxicity of blasts was carried out in AML patient and in normal bone marrow mononuclear cells(NBMMC).NBMMC from 11 healthy volunteers and AML blasts from 23 patients were incubated for 48h with HGF plus antileukemic drug.The drug sensitivity showed marked variation from case to case. The results showed enhancement of the cytotoxicity of DNR,VP 16 by GM CSF or IL 3. The results suggest significant enhancement of the H cytotoxicity for the AML samples by GM CSF.However,none of the HGF significantly effects the drug cytotoxicity of NBMMC.