四-(4-吡啶)卟啉分光光度法测定松花蛋中铅的含量

Determination on Pb Content in Preserved Duck Eggs by Four-(4-pyridine) Porphyrin Spectrophotometer

[目的]探讨四-（4-吡啶）卟啉与铅显色反应的最佳条件,以准确测定松花蛋中铅含量。[方法]测定和研究了pH值、温度、反应时间、共存离子、表面活性剂、试剂的用量等多因素对铅和四-（4-吡啶）卟啉的显色反应的影响,进而确定了反应的最适合条件,建立了一种新型的测定松花蛋中铅含量的分析方法。[结果]在十二烷基硫酸钠存在条件下,四-（4-吡啶）卟啉能与铅在碱性介质中生成稳定的配合物,配位比为1∶1 该配合物的最大吸收波长为464 nm,当铅含量在0~0.8 g/ml范围内呈线性关系 将其用于松花蛋中铅含量的测定,结果较为准确。[结论]在反应的最适合条件下建立的新型测定松花蛋中铅含量的分析方法具有方法简单、安全、结果比较准确的特点。

[ Objective ] The aim was to discuss the optimum condition for the color reaction of four-（4- pyridine） porphyrin and Pb so as to measure the Pb content in preservod.duck eggs. [ Method] The effect of pH value, temperature, reaction time, coexistent ions, surfactant, content of reagent etc. on the color reaction of Pb and four-（4- pyridine） porphyrin was measured and researched to make sure the optimum condition of reaction and establishe a new analysis method to measure the Pb content in preserved duck eggs. [ Result] Under the condition of existing sodium dodecyl sulfate, the stable complex of four-（4- pyridine ） porphyrin and Pb in the alkaline medium was generated, the complex rate was 1 ： 1. The greatest absorption wavelength of this complex was 464 nm and had a linear relation when the Pb content was in the 0 - 0.8 g/ml. The testing result was more accurate when the complex was used to measure Pb content in preserved duck eggs. [ Conclusion] The new analysis method of determining Pb content in preserved duck eggs was established under the optimum condition of reaction, which had the character of simple method, safety and accurate result.