红叶石楠组织培养技术比较研究

Study on the Techniques of Photinia fraseri Culture in vitro

[目的]研究红叶石楠组织培养技术。[方法]通过对红叶石楠外植体不同取样方式、不同增殖条件及不同生根方法的比较,研究其组织培养技术。[结果]结果表明,以休眠芽为外植体的红叶石楠的初始培养成功率较高,利用GA3浸泡消毒处理过的休眠芽,可促进其组织快速生长。培养基为50%MS、6-BA 1.00 mg/L、卡拉胶7.0 g/L时,红叶石楠组培增殖率最高。NAA直接生根培养,易产生愈伤组织,有效生根率最高为54.83%,IAA最高生根率可达57.83%。两步生根法愈伤组织形成少,NAA 3.0 mg/L+IAA 3.0 mg/L浸泡幼茎72 h,有效生根率达81.33%。[结论]该研究结果为红叶石楠组培技术的产业化应用提供参考。

[ Objective] The purpose of this research was to study the techniques of Photinia fraseri culture in vitro. [ Method] Ex-plant sampiing ways, muhiplying conditions and rooting methods of Photiniafraseri culture in vitro were researched. [ Result ] The results showed that after sterilized, dormant bubs dipped in GA3 for 24 hours, then cultured in initial media over 40 d, occurred more and longer aseptic shoots. The highest proliferation rate was resulted from 50% MS, 6-BA 1.00 mg/L and carageenan 7.0 g/L, and the more sub-cultured generations and the more multiplication rate increased during 2 - 8 generations. More callus and ineffective roots produced by NAA, among the treatments, the highest rooting percentage was 54.83% and 57.83%, respectively, at NAA 0.5 mg/L and IAA 1.5 mg/L, but there were 81.33% of the shoots had been induced normal roots by two steps rooting method： firstly, dipped the shoots in the solution of NAA 3.0 mg/L and IAA 3.0 mg/L for 72 h and then removed them to culture in media with no plant growth regulators. [ Conclusion ] This research results will provide the reference for industry applications of tissue culture technology, of Photinia fraseri.