大叶栎组织培养中外植体褐变的初步研究

Preliminary Study on Browning of Explant in Castanopsis fissa Tissue Culture

[目的]分析大叶栎多酚类物质含量对初代培养外植体褐变的影响情况。[方法]将大叶栎外植体接种在添加不同浓度的活性炭和抗坏血酸的MS培养基上,观察其褐变致死情况。同时对大叶栎不同年龄、不同部位试样的多酚类物质含量进行测定,分析其对初代培养外植体褐变的影响,初步研究降低大叶栎初代培养外植体褐变的措施。[结果]大叶栎不同器官、不同部位的试样多酚类物质含量不同,多酚类物质含量与大叶栎初代培养外植体褐变呈极显著的正相关（r=0.899）,在培养基中添加1.0~3.0 g/L活性炭和0.1~0.3 mg/L抗坏血酸有利于降低大叶栎初代培养外植体的褐变,活性炭的效果略优于抗坏血酸。[结论]大叶栎初代培养外植体的褐变与多酚类物质含量密切相关。

[ Objective ] The purpose was to analyze the effects of polyphenol substance content in Castanopsis fissa on explant browning in initial culture. [ Method] The browning caused death of C. fissa explant was observed by inoculating C. fissa explant on the MS medium with different concentration, of the active carbon and ascorbic acid. Meanwhile, the polyphenol substance contents in the samples of C. fusa with different ages in various positions were determined, its effects on explant browning of initial culture were analyzed and the measures of reducing explant browning in initial culture for C. fusa were researched preliminarily. [ Result] The polyphenol substance contents in the samples of C. fissa in different organs and different positions were varied, and the polyphenol substance contents had extremely significant positive correlation with explant browning in initial culture of C. fissa （ r = 0. 899）. Adding 1.0 - 3.0 g/L active carbon and 0. 1 - 0.3 mg/L ascorbic acid in medium was in favor of decreasing the browning of explant of C..fissa in initial culture, and the effect of active carbon was slightly better than that of ascorbic acid. [ Conclusion] The browning of explant of C. fissa in initial culture was closely related with polyphenol substance contents.