试验性趾叶炎小鼠肠组织中P物质的表达

Expression of Substance P in Intestinal Tissue of Experimental Mice with Digital Lobule Inflammation

[目的]为动物趾叶炎的预防和治疗提供免疫学基础。[方法]对昆明种小白鼠注射二磷酸组织胺制备趾叶炎小鼠病理模型,以注射生理盐水为对照。分别于给药后2、14、24、48、72 h及15、30 d取小鼠3~5 mm长的小肠组织块,检测肠组织中炎性细胞、杯状细胞、肥大细胞及其脱颗粒数、P物质表达水平的动态变化。[结果]趾叶炎小鼠的肠腺结缔组织内及其绒毛上P物质表达异常丰富,且P物质呈单根或片状走行,并与肥大细胞串联及相邻排列,随给药时间的延长,P物质表达逐渐增强,肥大细胞及其脱颗粒数也急剧增多 炎性细胞、杯状细胞、腺体数量与肥大细胞的变化趋势一致 给药72 h后,血管内壁出现裂隙,肠粘膜出现水肿。[结论]肥大细胞参与小鼠趾叶炎的发病过程,并影响小鼠肠粘膜的通透性。

[ Objective ] The aim was to provide the immunological basis for prevention and therapy of digital lobule inflammation. [ Method ] The Kunming white mice were injected with histamine diphosphate to prepare pathological model with digital lobule inflammation, with the treatment of injecting normal saline as CK. After 2, 14, 24, 48, 72 h and 15, 30 d of the administration, the 3 - 5 mm intestinal tissue mass were taken to detect the dynamic changes of inflammatory cells, goblet cells, mast cells and their degranulation number and the expression level of substance P. [ Result] The expression of substance P in intestinal gland connective tissue and on its villi of mice with digital lobule inflammation was abnormally rich. And substance P showed single or lamellar distribution and connected and neighboring arranged with mast cell. With the prolonging of administration time, the expression of substance P gradually increased, and the mast cells and their degranulation number dramatically increased. The changing trend of inflammatory cells, goblet cells and gland number was similar with that of mast cell. After 72 h of administration, the blood vessel wall appeared cracks and intestinal mucosa appeared edema. [ Conclusion ] Mast cells participated the pathogenic process of digital lobule inflammation of mice and influenced the permeability of intestinal mucosa.