mRNA差异显示研究胃癌相关基因

Studies on gastric cancer associated genes by mRNA differential display

为了克隆与胃癌发病相关的新基因，我们以正常胃粘膜细胞ＧＥＳ和胃癌细胞系７９０１为对比材料，利用ｍＲＮＡ差异显示技术，得到两个ｃＤＮＡ片段。经分析发现，分别与Ｔｒｋ基因及白血病病毒基因有８０％和３０％的同源性及较好的读框，有可能为胃癌相关的新基因片段，已在ＧｅｎＢａｎｋ中登录：１７６１１６，１７６３８５。

Cancer is a disease state caused by multiple genetic alterations which lead to uncontrolled cell proliferation. The process often involves the activation of cellular protooncogenes and inactivation of tumor suppressor genes. By using differential display, mRNAs from a normal gastric mucosa cell line GES and a gastric cancer cell line 7901 were compared to search novel gastric cancer associated genes. We report here the identification and characterization of two cDNA fragments designated GCC1 and GCC2, respectively, which were expressed exclusively in the gastric cancer cell line 7901. These sequences have been assigned the database ID numbers in GenBank: 176116, 176385.