66例大肠癌PCNA和CEA免疫组化检测分析

Immunohistochemical Detection of PCNA and CEA in 66 Cases of Large Intestine Carcinoma

采用ＬＳＡＢ免疫组化法检测６６例大肠癌中ＰＣＮＡ和ＣＥＡ的阳性表达。结果：ＰＣＮＡ阳性５７例（８６．４％），ＣＥＡ阳性５５例（８３．３％）。中等分化大肠癌与高分化大肠癌，其ＰＣＮＡ阳性表达有非常显著性差异（Ｐ＜０．０１），而ＣＥＡ阳性表达在大肠癌高、中和低分化组间有显著性差异（Ｐ＜０．０５）。ＰＣＮＡ和ＣＥＡ阳性表达在大肠癌分级中起到重要的作用。Ｄｕｋｅｓ′Ｂ期和Ｄｕｋｅｓ′Ｃ期，其ＰＣＮＡ和ＣＥＡ的阳性表达均有显著性差异（Ｐ＜０．０５）。结果表明ＰＣＮＡ和ＣＥＡ阳性表达的大肠癌，阳性强度越高，说明癌的分化程度越低，浸润性强，转移快。两者同时检测，起到互补作用，可作为估价预后的一个有效指标。

cases of large intestine carcinoma were studied using LSAB immunohistochemistry to detect proliferating cell nuclear antigen (PCNA) and carcinoembryonic antigen (CEA).Ressults:57 cases (86.4%) were positive for PCNA and 55 cases ( 83.3% ) were positive for CEA.The difference of PCNA positive rates had remarkable statistically significance between medial differentiated and poorly differentiated carcinoma ( P <0.01),as well as positive rate of CEA among three grades of the carcinoma.Between stages of Dukes′B and Dukes′C,the differences of postive rate of PCNA and CEA also had statistically significance( P < 0.05 ).A combinated detection of PCNA and CEA would be useful for indication of prognosis in large intestine carcinoma.