麝香保心丸对内皮祖细胞功能及分泌一氧化氮的影响

Effects of Shexiang Baoxin Pill on Function and Nitric Oxide Secretion of Endothelial Progenitor Cells

目的观察麝香保心丸(Shexiang Baoxin Pill)对人外周血内皮祖细胞(endothelial progenitor cells,EPCs)功能及分泌一氧化氮(NO)的影响。方法采用密度梯度离心法从外周血获得单个核细胞,将其接种在人纤维连接蛋白包被的培养板上,培养7天后收集贴壁细胞,加入不同浓度麝香保心丸继续培养24h;分别采用MTT比色法、Transwell小室、黏附能力测定实验、体外血管生成试剂盒和硝酸还原酶法测定EPCs的增殖、迁移、黏附、体外血管形成功能和分泌NO能力。结果与对照组比较,麝香保心丸组EPCs增殖能力提高,迁移、黏附和体外血管形成功能增强,培养上清中NO浓度增加(P<0.05),其作用随麝香保心丸浓度增加而增强。结论麝香保心丸显著改善EPCs功能,这可能是其内皮保护和促进心肌血管新生的新机制。

Objective To investigate the effects of Shexiang Baoxin Pill （SBP） on function of endothelial progenitor cells （EPCs） and its nitric oxide （NO） secretion. Methods Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and inoculated on the human fibro-ligandin encrusting plate. After 7 days of in vitro culture, adherent cells were collected and incubated with SBP for 24 h. The proliferation,migration,adhesive activity, vasculogenesis capacity and NO secretion of EPCs were assayed using MTT, Transwell chamber, adhesion determination, in vitro vasculogenesis kit and nitrate reductase method, respectively. Results EPCs incubated with SBP showed the capacities higher than those of control in proliferation, migration, adhesion, in vitro vasculogenesis, and with a higher NO concentration in the culture supernatant. Conclusion SBP can improve the function of EPCs, which might be a novel mechanism of its effects in improving vascular endothelial function and promoting angiogenesis.